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Showing: 9681 - 9700 of 28450 results

  4. pLC-Flag-SENP8 sg2R-P2A-Hygro

    Plasmid
    #124300
    Purpose
    Lentiviral vector for expression of Flag tagged SENP8-P2A-Hygro casette from a CMV promoter. SENP8 cDNA contains silent mutations to disrupt a sgRNA binding site.
    Depositor
    Eva Gottwein
    Article
    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.
    Insert
    SENP8 (SENP8 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    several silent mutations to disrupt sgRNA targeti…
    Promoter
    CMV
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    124300-plasmid-map-sequence-id-242478

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  7. pBT3038

    Plasmid
    #122545
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_glo-1::CemOrange2 (glo-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122545-plasmid-map-sequence-id-238133

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  8. pBT3043

    Plasmid
    #122546
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122546-plasmid-map-sequence-id-238140

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  12. PAK4-FL-3'UTR-MT2

    Plasmid
    #113093
    Purpose
    Luciferase reporter containing PAK4-FL- 3' UTR with the second predicted MiR-199a-3p binding site mutated
    Depositor
    James Donahue
    Article
    Phatak et al Oncotarget. 2018 Jun 19;9(47):28391-2840
    Insert
    p21 Activated kinase 4 (PAK4 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Second predicted miR-199a-3p binding site mutated…
    Promoter
    PGK
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    113093-plasmid-map-sequence-id-216990

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  14. KG#84

    Plasmid
    #110878
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA in ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    unc-17beta promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110878-plasmid-map-sequence-id-217951

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  15. pET-30a(+)-N-SH2+IA

    Plasmid
    #111273
    Purpose
    express murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk (N)SH2 domain plus interdomain A (Ser 8 to His 162), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
    Tags
    HHHHHHENLYFQG
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111273-plasmid-map-sequence-id-215706

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  16. KG#81

    Plasmid
    #110876
    Purpose
    Expresses the C. elegans acy-1 P260S gain-of-function cDNA in body wall muscle
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    myo-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110876-plasmid-map-sequence-id-212571

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  17. KG#83

    Plasmid
    #110877
    Purpose
    Expresses the C. elegans acy--1 P260S gain-of-function cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1
    Inserts
    rab-3 promoter
    acy-1 P260S gain-of-function cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Changed Proline to Serine at amino acid 260
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110877-plasmid-map-sequence-id-212517

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  19. LentiV_Neo_LKB1_CR

    Plasmid
    #108112
    Purpose
    Lentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance gene
    Depositor
    Christopher Vakoc
    Article
    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1
    Insert
    LKB1 (STK11 Human)
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    change cytosine 333 to alanine (silent mutation)
    Promoter
    EFS promoter
    Available Since
    April 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    108112-plasmid-map-sequence-id-207845

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  20. pDD2034

    Plasmid
    #101377
    Purpose
    Name: pBluescript-Δ611-843REV. Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV.
    Depositor
    Dirk Dittmer
    Article
    Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1
    Insert
    Blue Heron Biotechnology Inc. bidirectional mutant variant of pBluescript-LANApi,K14 (pDD2000), 611-843 REV
    Use
    Unspecified
    Mutation
    Blue Heron Biotechnology Inc. bidirectional mutan…
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    101377-plasmid-map-sequence-id-194529

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Showing: 9681 - 9700 of 28450 results