We narrowed to 27,202 results for: c-myc

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Publication

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Expression

Plant

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Publication

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pH-Ubi-miniSdd7-nCas9-PBE

Plasmid

#204856

Purpose

For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Publication

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1, OsU3, 35S

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pYZ463

Plasmid

#187971

Purpose

TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

Depositor

Jef Boeke

Publication

Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

Insert

Cas9 (codon optimized for human)

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Flag- MCL1-3A (S159, S162, T163 mutated to A)

Plasmid

#197458

Purpose

MCL-1 phosphodegron mutants

Depositor

Omar Khan

Publication

Keyan et al Cancers (Basel). 2023 Jan 13;15(2):505.

Insert

MCL1 (MCL1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S159A, S161A, T163A

Promoter

CMV

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEntry_HCoV-229E-Spike

Plasmid

#168971

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Spike (S )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Publication

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight1

Plasmid

#198739

Purpose

Expresses psychLight1 in mammalian cells

Depositor

Lin Tian

Publication

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsEDC4_S

Plasmid

#147455

Purpose

Mammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.

Depositor

Elisa Izaurralde

Publication

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsEDC4 (EDC4 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCK007 pME KalTa4

Plasmid

#195965

Purpose

Gateway pME Gal4 variant

Depositor

Alexa Burger, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

KalTA4 (Gal4)

Use

Other

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PG13-mCherry-EF1a-PuroR-EGFP

Plasmid

#195292

Purpose

piggyBac transposon vector with mCherry reporter containing 13 copies of the p53-binding consensus sequence (constitutive puromycin resistance and EGFP)

Depositor

Hideyuki Oguro

Publication

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

mCherry
PuroR-P2A-EGFP

Use

Piggybac transposon

Expression

Mammalian

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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SFFV-HALO-CTERM-GFP

Plasmid

#185767

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, Halo

Mutation

none

Promoter

SFFV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PGK-AID-NTERM-GFP

Plasmid

#185773

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Publication

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, AID

Mutation

none

Promoter

PGK

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_ccdB_lox

Plasmid

#183763

Purpose

Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

ccdB

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Tags

FRB-mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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σ1-mCherry

Plasmid

#186578

Purpose

Expression of adaptor protein 1 (AP1) sigma subunit AP1S1 tagged with mCherry

Depositor

Stephen Royle

Publication

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP1S1 (AP1S1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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cyt-GafD-mTurboID-V5

Plasmid

#184640

Purpose

Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

Depositor

Charlie Fehl

Publication

Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

Insert

cyt-GlycoID-V5

Tags

Nuclear export sequence and V5 tag

Expression

Mammalian

Mutation

GafD -> expressed aa22-178 ; BirA* -> expre…

Promoter

CMV

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Publication

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLacO-pEF1α-RFP

Plasmid

#179507

Purpose

Expresses monomeric RFP in mammalian cells under a EF1α promoter

Depositor

Yasushi Hiraoka

Publication

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

monomeric RFP

Expression

Mammalian

Promoter

EF1α

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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