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We narrowed to 35 results for: c-myc

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Showing: 1 - 20 of 35 results

  1. Of Myc and Men

    Type
    Blog Post
    ...bound to the C-terminal peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10])....probe the functions of the human c-Myc gene (Evan, et al. 1985). Myc was one of the earliest identified...sequences within the middle of the human c-Myc protein or the C-terminus, Evan, et al. isolated six different...Myc1-9E10 and the other C-terminal antibodies only recognized the human c-Myc protein and did not recognize...when Evan’s team generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new...Addgene.org  Find recombinant anti-c-Myc [9E10] at Addgene Find plasmids with the myc-tag  ...the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into those questions in this post...

  2. Hot Plasmids - October 2022

    Type
    Blog Post
    ...on the Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here...light-gated potassium channelrhodopsins Myc-tag antibody anti-c-Myc [9E10] now available! Improved voltage...doi.org/10.1101/2021.09.17.460684.)   Myc-tag antibody anti-c-Myc [9E10] now available! by: Ashley Waldron.... It is derived from the C-terminal region of the human proto-oncogene c-Myc. Since the generation of ...of the first anti-c-Myc [9E10] (Evan, et al., 1985), the Myc tag has become one of the most commonly used... for ‘Myc tag’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10...Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Molecular and Cellular Biology...

  3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    ...enhances iPSC generation as does replacing C-MYC with L-MYC [10, 11]. While the mechanisms underlying ...addition of an shRNA for P53 and replacing C-MYC with L-MYC in the episomal reprogramming cocktail by ... reprogramming factors- OCT3/4, SOX2, KLF4, and C-MYC [1]. For the derivation of clinical grade hiPSCs... that encoded six factors - OCT3/4, SOX2, KLF4, C-MYC, LIN28 and SV40 large T antigen (SV40LT). The method...pCXLE-hSK 27078 SOX2 and KLF4 pCXLE-hUL 27080 L-MYC and LIN28 pCXLE-hOCT3/4-shp53-F 27077 OCT3/4 and...direct reprogramming by transformation-deficient Myc. Proc Natl Acad Sci U S A, 2010. 107(32): p. 14152...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...state using a cocktail of factors (Oct3/4, Sox2, c-Myc, and Klf4) that are known to maintain pluripotency...Doxycycline-inducible expression of human Oct4, Sox2, Klf4, and c-Myc from four separate lentiviral plasmids A drug-inducible...for the expression of human Oct4, Klf4, Sox2, and c-Myc Human Induced Pluripotent Stem Cells Produced Under... vector for the expression of human Oct4, Klf4, c-Myc, and Sox2 Integrative Analyses of Human Reprogramming...Lentivirus Human Expression of human Klf4, Oct4, c-Myc, and Sox2 as VP-16 transcriptional activating fusions...polycistronic expression of human Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53 for single plasmid reprogramming...retroviral expression of human Sox2, Oct3/4, Klf4, and c-Myc from four separate plasmids Induction of pluripotent...

  6. Plasmids 101: Protein tags

    Type
    Blog Post
    ...to it. The FLAG, hemaglutinin antigen (HA), and c-myc tags have been the workhorses of the affinity tag... tags, a His tag can be fused to either the N- or C-terminus of a protein. Unlike other epitope tags –...step purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western...

  7. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...-RFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes...pTag-BFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagBFP James Johnson 79800 pTag-RFP-C-h-Rab4a-c-Myc Endosomes (recycling...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-...-BFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagBFP James Johnson 231781 pCMV-mGold2t-Rab4a-C-7 Endosomes...pCMV-mGold2t-CAF1-C-10 Nucleus CAF-1 mGold2t Francois St-Pierre 231774 pCMV-mGold2s-CAF1-C-10 Nucleus CAF...pCMV-mGold2t-Actin-C-18 Actin filaments beta-Actin mGold2t Francois St-Pierre 231772 pCMV-mGold2s-Actin-C-18 Actin...pCMV-mGold2t-Tubulin-C-18 Microtubules alpha-Tubulin mGold2t Francois St-Pierre 231771 pCMV-mGold2s-Tubulin-C-18 Microtubules...

  8. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...four reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert...additional pluripotency stage) Less Safety Less (C-MYC, a known oncogene is one of the reprogramming factors...22645348. PubMed Central PMCID: PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth... PubMed Central PMCID: PMC4363833. 35. Eguizabal, C., et al., Dedifferentiation, transdifferentiation,...1): p. 82-94. PubMed PMID: 23329641. 36. Jopling, C., S. Boue, and J.C. Izpisua Belmonte, Dedifferentiation...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived...Epitope tags are short peptides introduced at the N or C terminus of a protein that are bound by antibodies...enterokinase, which recognizes the 5 amino acids on the C terminal of the tag. Of the most common tags, FLAG...negatively charged and 1 positively charged amino acids. Myc tags are frequently used for western blot, flow cytometry... However, protein purification is not ideal with Myc, as low pH conditions are required during the purification...affect protein function. Fun fact: The antibody to Myc was developed in 1985 and is still the most prevalent...Addgene has you covered with monoclonal antibodies to Myc for all of these species. HA The HA tag is a 9 amino...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...display vector with a C-terminal Myc tag pPMW-attB - pUASp plasmid with N-terminal Myc tag and attB for Drosophila...transgenesis His Epitope tag pEZYmyc-His - C-terminal Myc-His tag for mammalian expression (Gateway... Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA...pNIC-CTHF - C-terminal TEV-His6-Flag for bacterial expression pFA6a vectors - PCR-based C-terminal...pENTR vector for C-terminal 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc...- N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector for Myc tag pETcon(-)...backbone, mammalian expression pENTR4-myc-nuc - pEntry vector that adds a C-terminal nuclear localization signal...

  13. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq data...several MYC-dependent cancer cell lines. It can be applied for identification of essential MYC binding... system is capable of robust expression of high G+C content genes, as validated through monitoring the...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model for reprogramming somatic...fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, ...coselection, analogous to methods previously used in C. elegans, targets the sodium/potassium pump ATP1A1...article have been deposited with Addgene and include MYC- and FLAG-tagged TZAP constructs (wild-type and truncation...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...five transcriptional reporters for NF-kb, TGF-b, c-Myc, p53, and MAPK/JNK plus a constitutive control. ...87075 pLenti6.2-ccdB-Nanoluc NanoLuc® Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival...Alberto Macho 174051 pGWB-cLUC Firefly Creation of C-terminal Fifrefly luciferase fragment for split-luciferase...Mammalian expression of Nanoluc with a N-terminal Myc tag Erich Wanker 124701 pLenti-PGK-Venus-Akaluc (...Lentiviral expression of Nanoluc with a N-terminal Myc tag Erich Wanker 115352 pFL-SV40 Firefly SV40 Mammalian...fusing NanoLuc® and Venus fluorophore to Troponin C Ca 2+ binding domain. Luminopsins : Luciferase-opsin...

  16. Addgene Research Program

    Type
    Collection
    ...well-characterized epitope tag antibodies (Anti-c-Myc [9E10] for mouse and Anti-HA [12CA5] for rat) into...backbones are available untagged or with an N- or C-terminal hemagglutinin (HA) tag to aid with protein...

  17. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...protocol starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the...due to camelid high body temperature (37.2 - 39.2°C). The Seeger lab found a way around this by using ...the expression vector pSb-Init, which attaches a Myc-tag and a His6-tag to the end of each sybody candidate...

  18. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...expression ~ ~ ~ ~ ✓   ✓ SV40 and c-Myc nuclear localization sequences Improve translocation...j.cell.2021.09.018 Choi, J., Chen, W., Suiter, C. C., Lee, C., Chardon, F. M., Yang, W., Leith, A., Daza,..., J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A.,...Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase (PE1). But innovations...pegRNAs (Yan et al., 2024). The team fused La to the C-terminal end of PEmax to produce PE7. PE7 significantly...References Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy... Koblan, L. W., Levy, J. M., Chen, P. J., Wilson, C., Newby, G. A., Raguram, A., & Liu, D. R. (2019). ...

  19. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCMVTNT PINK1 N-myc PINK1 Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's...Strep-Lrrk2-Myc LRRK2 Myc, Strep CMV Parkinson's Maik Hintze 161583 pPuro3.1(+)_Strep-Lrrk2-Myc LRRK2 Myc, Strep...James Trimmer 200727 pLenti-C-Myc-DDK-IRES-Puro HNRNPA1 HNRNPA1 Flag, Myc CMV ALS Claudio Alarcon 200789...LRRK2-WT LRRK2 His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted ... pGW1-Myc-DJ1-WT PARK7 V5 CMV Parkinson's Mark Cookson 29349 pGW1-Myc-DJ1-L166P/K130R PARK7 Myc CMV Parkinson's...

  20. Hot Plasmids: May 2026

    Type
    Blog Post
    ...fraction of the price; you can choose 10xHis-, MBP-, or myc-tagged SerMNucA, depending on your preference or ...SerMNucA (Benzonase/Turbonuclease) as 10xHis, MBP or myc-tag fusions. protocols.io, doi: https://dx.doi.org...overview image) and 20 µm (magnification images). C,D) Mean ± s.e.m. signal z-score from PinkyCaMP and...nLightG2 or nLightG2-ctr in baseline (BL) session (C) or re-exposure (RE) session after fear conditioning...
Showing: 1 - 20 of 35 results