We narrowed to 26,411 results for: lars

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - H

Plasmid

#229972

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - H

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2AmcherryP2AP -TagBFPH

Plasmid

#229971

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2AmcherryP2AP -TagBFPH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

Plasmid

#231568

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

All-in-one LOOMINA construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_i1

Plasmid

#231416

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-1.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper TR G414C

Plasmid

#207612

Purpose

U3 promoter driven expression of the telomerase RNA with a G414C CAB box mutation

Depositor

Jens Schmidt

Article

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

Telomerase RNA G414C

Expression

Mammalian

Promoter

U1

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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aTY173_pPGK1_mTagBFP2_WPRE

Plasmid

#225352

Purpose

Lentiviral vector for BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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BALF5-D755N-D757N

Plasmid

#192455

Purpose

Expresses BALF5 with D755N and D757N mutations

Depositor

Bill Sugden

Article

Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

Insert

BALF5 (D755N&D757N) (BALF5 Epstein-Barr Virus)

Tags

FLAG

Expression

Mammalian

Mutation

D755N & D757N

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V1

Plasmid

#226538

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V2

Plasmid

#226539

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V2

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V2

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V3

Plasmid

#226540

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X5

Plasmid

#226547

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X5

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X7

Plasmid

#226549

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X7

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X7

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X1

Plasmid

#226543

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X1

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap V5

Plasmid

#226542

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD V5

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap V5

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swap X4

Plasmid

#226546

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X4

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X4

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDest17_A1-LCD swapX3

Plasmid

#226545

Purpose

Bacterial expression of N-terminally 6His tagged A1-LCD X3

Depositor

Tanja Mittag

Article

Pesce et al Sci Adv. 2024 Aug 30;10(35):eadm9926. do

Insert

A1-LCD swap X3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

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