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pAJT243-Pcin

Plasmid

#202301

Purpose

PCin-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pcin-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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plYJP067-Pttg

Plasmid

#202300

Purpose

PTtg-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTtg-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT238-Pbad

Plasmid

#202298

Purpose

PBad-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

Pbad-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT237-Ptac

Plasmid

#202297

Purpose

PTac-YFP plasmid for LPattB2 integration

Depositor

Christopher Voigt

Article

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

PTac-YFP

Expression

Bacterial

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22y/PfPRMT5-PTP

Plasmid

#203160

Purpose

for tagging PTP to the 5' end of PfPRMT5 gene by single crossover

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment, PTP tag and pDT 3' UTR

Tags

3' UTR of P. berghei dihydrofolate reductase…

Expression

Mammalian

Promoter

No

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3

Plasmid

#201534

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB3

Use

Synthetic Biology

Mutation

rhaS W190G

Promoter

PrhaBAD (rhamnose)

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP1

Plasmid

#203835

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 upstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.11_Nued2_E1_dAP2

Plasmid

#203836

Purpose

Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

dAP1

Expression

Mammalian

Mutation

KO of AP1 downstream

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2trLATo

Plasmid

#203746

Purpose

Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trLATo (Lat Mouse)

Tags

ER translocation signal - mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_E30_VP1

Plasmid

#202070

Purpose

Bacterial expression of VP1 protein from echovirus 30 (E30). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

E30 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_CVB1_VP1

Plasmid

#202069

Purpose

Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB1 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_CVA4_VP1

Plasmid

#202068

Purpose

Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVA4 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

Plasmid

#201533

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB2

Use

Synthetic Biology

Mutation

rhaR L128+1bp (frameshift), ilvA G455C

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

Plasmid

#201535

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB4

Use

Synthetic Biology

Mutation

rhaS ΔM1-H5, PrhaSR Δ179bp

Promoter

PrhaBAD (rhamnose)

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-domain

Plasmid

#200300

Purpose

Bacterial expression plasmids for production of recombinant beta-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_alpha-domain

Plasmid

#200302

Purpose

Bacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

alpha-domain of metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC28-HiBIT-WDR5

Plasmid

#200883

Purpose

Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-WDR5 (WDR5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1149-1376_AC

Plasmid

#148475

Purpose

Insect Expression of DmNot1_1149-1376

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1149-1376 (Not1 Fly)

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HsNot1iso1_1318-1605-HA-MBP_AD

Plasmid

#148503

Purpose

Mammalian Expression of HsNot1iso1_1318-1605

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605 (CNOT1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-R249H_X

Plasmid

#147979

Purpose

Insect Expression of DmCaf40-R249H

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-R249H (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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