Addgene

We narrowed to 25,793 results for: sta

Showing: 9921 - 9940 of 25793 results

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J23101

Plasmid

#55176

Purpose

Very weak constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

J23101 promoter

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

J23101

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC3

Plasmid

#72712

Purpose

Lentiviral expression of shRNA against CRTC3 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC3

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCS5

Plasmid

#55750

Purpose

Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Use

Tags

Expression

Bacterial

Mutation

Residues 59-264 of KanR

Promoter

T7-lac

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pEF HA-TSAdwt

Plasmid

#84517

Purpose

Mammalian expression of TSAd

Depositor

Anne Spurkland

Article

Granum et al BMC Immunol. 2006 Jul 13;7:15.

Insert

TSAd (SH2D2A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
px330-UFSP2 sgRNA2

Plasmid

#134637

Purpose

contains sgRNA targeting human UFSP2 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFSP2 sgRNA2 (UFSP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDIRECT_10A

Plasmid

#91207

Purpose

Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:CheRiff-tdTomato

Plasmid

#124234

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CheRiff-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST mito roGFP2-Orp1

Plasmid

#64997

Purpose

for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Use

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMC-6-GFP-P2A-NptII-9

Plasmid

#197741

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EGFP-P2A-NptII bicistronic CDS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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R10-3 /pQE-30

Plasmid

#79859

Purpose

The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.

Depositor

Konstantin Lukyanov

Article

Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73

Insert

R10-3

Use

Tags

HisTag

Expression

Bacterial

Mutation

Aequorea victoria green fluorescent protein (avGF…

Promoter

T5 promoter / lac operator

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b – E. coli RecE residues 564-868

Plasmid

#104534

Purpose

Expresses E. coli RecE residues 564-868

Depositor

Charles Bell

Article

Zhang et al Structure. 2009 May 13;17(5):690-702. do

Insert

RecE residues 564-866 (C-terminal nuclease domain)

Use

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-4SA(S164)

Plasmid

#33083

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S61A, S109A, S127A, S381A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Use

Tags

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pDT-sgRNA-XMAS-2x

Plasmid

#164414

Purpose

Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

Depositor

Xiao Wang

Article

Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

Insert

sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9 GW STOP

Plasmid

#183196

Purpose

Destination vector

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRRG199-bmp4

Plasmid

#99076

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Bmp4 (also known as bmp) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-Gdf7

Plasmid

#163597

Purpose

Expresses GDF-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf7 (Gdf7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Some Guanin and Cytosine were substituted to redu…

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMOD_A1810

Plasmid

#91040

Purpose

Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9_dead (D10A + H840A)

Use

CRISPR

Tags

Expression

Mutation

D10A, H840A

Promoter

ZmUbi

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only