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His6-Twin-Strep-SUMO-pET28a-EfaCas2

Plasmid

#112794

Purpose

To express enterococcus faecalis Cas2 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas2

Tags

His6-Twin-Strep-SUMO

Expression

Bacterial

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6630

Plasmid

#106160

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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XMRV vp62

Plasmid

#26189

Depositor

Joseph DeRisi

Article

Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

Insert

XMRV VP62 (gag Human)

Expression

Mammalian

Available Since

Nov. 16, 2010

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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Galt1-sCGrx1p-HA

Plasmid

#230028

Purpose

Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

Depositor

Pablo Rivera-Fuentes

Insert

Galt1-sCGrx1p-HA (B4GALT1 Synthetic, Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pMTIKA

Plasmid

#22061

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

mCMV-TetOn expression cassette

Expression

Mammalian

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-ar1

Plasmid

#225219

Purpose

Expression androgen receptor 1 in mammalian cells

Depositor

Deshou Wang

Article

Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

Insert

androgen receptor 1

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAV0620

Plasmid

#133506

Purpose

Expression of eGFP tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-eGFP-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKMV-VioB

Plasmid

#65348

Purpose

systhesis of vioB gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioB

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Calreticulin (CALR Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
c-myc promoter (4x TBE1 - wt)

Plasmid

#16597

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE1 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pHR pUAS-dGFP pPGK-iRFP

Plasmid

#176530

Purpose

Lentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.

Depositor

Jared Toettcher

Article

Ravindran et al Cell Syst. 2022 Feb 16;13(2):131-142.e13

Inserts

dGFP
iRFP

Use

Lentiviral

Expression

Mammalian

Promoter

PGKp and UASp

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK527

Plasmid

#110605

Purpose

BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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px458_2A_GFP_sgRNA_TRIM25_G1

Plasmid

#127119

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA TRIM25 (TRIM25 Human)

Use

CRISPR

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMP4657

Plasmid

#107773

Purpose

Expression of EGFP

Depositor

Herman Spaink

Article

Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

pLacZ-EGFP in pME6010

Tags

pLacZ-EGFP

Expression

Bacterial

Promoter

pLacZ

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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eGFP-CBP

Plasmid

#169211

Purpose

Expression of eGFP-CBP protein for chitin visualization

Depositor

James S Fraser

Article

Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

Insert

Chitinase A1 Chitin Binding Domain

Tags

6xHis, Mxe Gyr Intein, and eGFP

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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