We narrowed to 14,477 results for: NTS

1. pDONR223_PDZD11_p.R133H

   Plasmid

   #81264

   Purpose

   Gateway Donor vector containing PDZD11 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   PDZD11 (PDZD11 Human)
   

   Use

   Gateway entry vector

   Mutation

   R133H, R137W

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_REG1A_p.S131I

   Plasmid

   #81265

   Purpose

   Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   REG1A (REG1A Human)
   

   Use

   Gateway entry vector

   Mutation

   S131I

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_KLRC2_p.G174R

   Plasmid

   #81270

   Purpose

   Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KLRC2 (KLRC2 Human)
   

   Use

   Gateway entry vector

   Mutation

   N2S, F102S, G174R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cry2 mcherry Gamma 9 in pcDNA3.1

   Plasmid

   #64208

   Purpose

   mCherry fused between Cry2 and gamma 9 to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 (CRY2 Mustard Weed)
   gamma cone (GNGT2 Human)
   

   Tags

   mcherry and n/a

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

5. KHBD00021

   Plasmid

   #34183

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG14548 (E(spl)mbeta-HLH Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223_CDK4_p.R24C

   Plasmid

   #82175

   Purpose

   Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Gateway entry vector

   Mutation

   R24C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-dCas9-p300-P2A-PuroR

   Plasmid

   #83889

   Purpose

   Lentiviral Sp dCas9-p300-P2A-PuroR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
   pac from Streptomyces alboniger
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PEmax-P2A-hMLH1dn

   Plasmid

   #174828

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   PEmax-P2A-hMLH1dn
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript; deletion of residues 754-…

   Promoter

   CMV

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid

    #73205

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
    

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_ERN1_WT

    Plasmid

    #82115

    Purpose

    Gateway Donor vector containing ERN1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ERN1 (ERN1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_CD70_p.D165Y

    Plasmid

    #81603

    Purpose

    Gateway Donor vector containing CD70 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CD70 (CD70 Human)
    

    Use

    Gateway entry vector

    Mutation

    D165Y

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCmini-iCAP-AAV.CAP-B10

    Plasmid

    #175004

    Purpose

    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY1052 PASTEv3 pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Plasmid

    #179105

    Purpose

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN3-RT(L139P)-(GGS)6-BxbINT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PEmax-P2A-BSD

    Plasmid

    #174821

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-BSD marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid

    #140003

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid

    #229678

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold, HA

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid

    #229679

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    monomeric StayGold

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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