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DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A 1-1327

Plasmid

#241253

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-1327 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-1327 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A 1-1364

Plasmid

#241254

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-1364 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-1364 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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PX458 BLTP3A knockout

Plasmid

#241256

Purpose

Knock-out plasmid targeting the second exon of human BLTP3A (UHRF1BP1)

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) KO gRNA (BLTP3A Human)

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3A 1-336

Plasmid

#241250

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) 1-336 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) 1-336 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3 chimera-1

Plasmid

#241251

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) chimera-1 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) chimera-1 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmCh-N1 BLTP3 chimera-2

Plasmid

#241252

Purpose

For exogenous expression of codon optimized BLTP3A (UHRF1BP1) chimera-2 as a C-terminal fusion to RFP in mammalian cells

Depositor

Pietro De Camilli

Article

Hanna et al EMBO J (2025) 44: 6168 - 6195

Insert

BLTP3A (UHRF1BP1) chimera-2 (BLTP3A Human)

Tags

RFP

Expression

Mammalian

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-Nterm

Plasmid

#242942

Purpose

Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Nterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-C-term

Plasmid

#242943

Purpose

Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Cterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway Cloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMEF2c-AHF-DreERT2 (JDW 196)

Plasmid

#242586

Purpose

The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Expression

Mammalian

Promoter

mouse Mef2c

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway Cloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mBaoJin-WPRE (JDW 1383)

Plasmid

#242549

Purpose

Gateway middle entry clone containing H2B-mBaoJin with WPRE at the 3' end; Nuclear green fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mBaoJin

Use

Gateway Cloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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GB3471

Plasmid

#215244

Purpose

CDS of HispS from N. nambi codon optimized for N.benthamiana. This gene is the first step of the bioluminiscente pathway.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

HispS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG005

Plasmid

#231309

Purpose

Vector part for BEVA; BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 3 RSF1010 broad host range origin of replication with oriT, CmR.

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG021

Plasmid

#231305

Purpose

Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 1 Level 1 BsaI cloning site without T0, AmpR

Expression

Bacterial

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG001

Plasmid

#231307

Purpose

Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Insert

BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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