We narrowed to 418 results for: px458

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px458_2A_GFP_sgRNA_ELAVL2

Plasmid

#106107

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA targeting ELAVL2 (ELAVL2 Human)

Use

CRISPR

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_2

Plasmid

#104049

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_1

Plasmid

#104048

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RXRB_1

Plasmid

#104050

Purpose

Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RXRB gRNA (RXRB Human)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_2

Plasmid

#86300

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MBD1_iso1_2

Plasmid

#86304

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPA_2

Plasmid

#86359

Purpose

Encodes gRNA for 3' target of human CEBPA

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against CEBPA (CEBPA Human)

Use

CRISPR

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_2

Plasmid

#86347

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KLF9_1

Plasmid

#86346

Purpose

Encodes gRNA for 3' target of human KLF9

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KLF9 (KLF9 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CEBPG_1

Plasmid

#86344

Purpose

Encodes gRNA for 3' target of human CEBPG

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against CEBPG (CEBPG Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TEAD3_1

Plasmid

#86337

Purpose

Encodes gRNA for 3' target of human TEAD3

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against TEAD3 (TEAD3 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_2

Plasmid

#86326

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HHEX_1

Plasmid

#72354

Purpose

Encodes gRNA for 3' target of human HHEX along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Insert

HHEX (HHEX Human)

Use

CRISPR

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_1

Plasmid

#73533

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_2

Plasmid

#73534

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
PX458-3xHA-en1FnCas9

Plasmid

#201947

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en1FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1369R on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX458_GAPDH-sgRNA

Plasmid

#249575

Purpose

Expresses SpCas9, GFP, and sgRNA targeting GAPDH.

Depositor

Joe Zhang

Publication

Zhang et al STAR Protoc. 2025 Jul 4;6(3):103928. doi

Insert

GAPDH-sgRNA

Use

CRISPR

Promoter

U6 promoter

Available since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_3

Plasmid

#73531

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX458-sgRNA_Ago2_4

Plasmid

#73532

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Publication

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AGO2

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-GFP (PX458)

Plasmid

#129025

Purpose

targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

Depositor

Julia K Polansky

Publication

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-huTET1CD, SgRNA cloning site

Tags

HA-Tag, NLS and T2A-EGFP

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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