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pDpsTE-Ypet

Plasmid

#98277

Purpose

mammalian expression of DpsTE-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

Dps

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Use

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP6

Plasmid

#71469

Purpose

Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 6 (SENP6 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET32a-hAREG

Plasmid

#199229

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Amphiregulin preproprotein (AREG Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a Hbb

Plasmid

#26639

Depositor

Phoebe Rice

Article

Mouw et al Mol Microbiol. 2007 Mar . 63(5):1319-30.

Insert

Hbb (hbb Borrelia burgdorferi)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET21a - Mtb-TF

Plasmid

#111513

Purpose

Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

Trigger Factor

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Use

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-HA-CPDSalI

Plasmid

#38254

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a-UVRAG CC

Plasmid

#24400

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

UVRAG (UVRAG Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains on the CC domain, amino acids 200-270

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Use

Tags

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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PCx35IL-pET42

Plasmid

#42916

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 35 cytoplasmic loop

Use

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 101-179

Promoter

T7

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET3_HMV-His6

Plasmid

#164843

Purpose

Expression of FL human metavinculin

Depositor

Tina Izard

Article

Rangarajan et al PLoS One. 2010 May 19;5(5):e10679. doi:

Insert

VCL (VCL Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_S-Luc_CLIP

Plasmid

#113923

Purpose

Plasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

S-Luc_CLIP

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-HA-CPDSalI

Plasmid

#38253

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pET23-CBD

Plasmid

#30311

Depositor

Klaus Hahn

Insert

CBD (WAS Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pET30-2-GPD1L

Plasmid

#83912

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET31b-T7-Spinach2-Dp

Plasmid

#79159

Purpose

Expresses the Dp biosensor under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

T7-tRNA-Spinach2-Dp

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Ubp8/PET32a

Plasmid

#26965

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Ubp8 (UBP8 Budding Yeast)

Use

Tags

His6 and Trx

Expression

Bacterial

Mutation

TEV site is added t the 5' end and 4 alanine…

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only