We narrowed to 11,594 results for: EMB

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p3E-WPRE-bGH-pA (JDW 1221)

Plasmid

#224527

Purpose

A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

WPRE-bGH

Use

Gateway Cloning

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

Plasmid

#224491

Purpose

Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Golgi-mTagBFP-P2A-H2A-tdiRFP

Use

Gateway Cloning

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GB3774

Plasmid

#215247

Purpose

CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CPH

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-hs_Cldn5 (JDW 1120)

Plasmid

#224481

Purpose

Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Claudin-5 promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

Plasmid

#224537

Purpose

A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

IRES-(n2)StayGold(c4)-pA

Use

Gateway Cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-FKBP12-Sm

Plasmid

#217004

Purpose

Expression of FKBP12-Linker-Small BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

FKBP12-Linker-Small BiT

Tags

Split Nanoluciferase SmallBiT

Expression

Bacterial

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-Lg-B

Plasmid

#216960

Purpose

Expression of Large BiT-Linker-GvpB

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Large BiT-Linker-GvpB

Tags

FLAG-Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-B-Lg

Plasmid

#216959

Purpose

Expression of GvpB-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

GvpB-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT-FLAG

Expression

Bacterial

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1 (C159A/C219A)

Plasmid

#224751

Purpose

In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

C159A/C219A

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2(S156C)

Plasmid

#224770

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2(S156C)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S156C

Promoter

T7

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/K271Q)

Plasmid

#224750

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/K271Q)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / K271Q

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/G171F)

Plasmid

#224749

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/G171F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / G171F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMHE:mTREK-1(S131C/A286F)

Plasmid

#224748

Purpose

In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Use

In vitro transcription for oocyte expression

Mutation

S131C / A286F

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pST39-Mega'

Plasmid

#216877

Purpose

Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

Derived from Mega, and GvpS and GvpK overlap has been resolved

Expression

Bacterial

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET26b(modified)-FRB-Lg

Plasmid

#216981

Purpose

Expression of FRB-Linker-Large BiT

Depositor

George Lu

Article

Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

Insert

FRB-Linker-Large BiT

Tags

Split Nanoluciferase LargeBiT

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-BAF

Plasmid

#222414

Purpose

To express BAF with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

BAF (BANF1 Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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GB3474

Plasmid

#215370

Purpose

CDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathway

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CPH

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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GB3473

Plasmid

#215246

Purpose

CDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathway

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

Luz

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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GB3472

Plasmid

#215245

Purpose

CDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathway

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

H3H

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkB ZU5ex-3xHA

Plasmid

#213732

Purpose

Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkB ZU5ex (Ank2 Mouse)

Expression

Mammalian

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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