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pFH2.32_LV-mCherry-MS2x8-BCS

Plasmid

#205539

Purpose

Express mCherry-MS2x8 with barcode

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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5n1 (560)

Plasmid

#70730

Purpose

TetR/PhlF/SrpR/LambdaCI/LacI BCD7 5-node v41-2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Inserts

srpR-ssrA
lambdaCI-ssrA
phlF-ssrA
lacI-ssrA
tetR-ssrA

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-NLRP10

Plasmid

#38141

Depositor

Thomas Kufer

Article

Lautz et al Cell Microbiol. 2012 Jun 4. doi: 10.1111

Insert

NLRP10 (NLRP10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pBAD-LSSmCherry1

Plasmid

#89986

Purpose

Long stokes-shift variant of red fluorescent protein mCherry

Depositor

Robert Campbell

Article

Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

Insert

LSSmCherry1

Tags

6xHis-T7-Xpress

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV-EF1s HA.NLS.Sce(opt)

Plasmid

#31479

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV/Ef1s HA NLS I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

"opt" indicates the coding sequence has…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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CMV-enAsCas12f

Plasmid

#204637

Purpose

Expression of enAsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

enAsCas12f

Tags

NLS

Expression

Mammalian

Mutation

D196K, N199K, G276R, N328G, D364R

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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mycUBB+1

Plasmid

#20254

Depositor

Nico Dantuma

Article

Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Insert

Ubiquitin B +1 transcript (UBB Human)

Tags

myc

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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mCarmine in pRSETB

Plasmid

#109484

Purpose

bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

Depositor

Oliver Griesbeck

Article

Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

Insert

mCarmine

Tags

N-terminal histidine tag in vector

Expression

Bacterial

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N

Plasmid

#80930

Purpose

Expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ135B2.0

Plasmid

#167159

Purpose

Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-GFP-LAA

Plasmid

#69928

Purpose

Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-GFP-LAA

Use

Synthetic Biology

Expression

Bacterial

Promoter

PlLacO1

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pHBJT034

Plasmid

#225180

Purpose

Low copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Tags

mCherry

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ136B2.0

Plasmid

#167160

Purpose

Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDV02

Plasmid

#212156

Purpose

Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Article

Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

Insert

secondary alcohol dehydrogenase

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_221

Plasmid

#91115

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Dbp

Plasmid

#186822

Purpose

Fluorescent reporter for Dbp expression

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

dbp promoter (Dbp Mouse)

Use

Luciferase

Promoter

dbp promoter

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCE048-SiT-Cas12a

Plasmid

#128124

Purpose

Express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-luxGold

Plasmid

#229948

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mGold fusion bioluminescent probe (max. Em=530nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxGold

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEC3135 (pERASE)

Plasmid

#220306

Purpose

Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pX330S-4

Plasmid

#58780

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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