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pDest-302 (attR4-attR2)

Plasmid

#161894

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-669 (attR4-attR3)

Plasmid

#161887

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTU-A-005

Plasmid

#124412

Purpose

Slot A, contains mKO2-MarA (step 1)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A with mKO2-MarA reporter + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pGDP1 vph

Plasmid

#112893

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Publication

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

vph

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAWp92

Plasmid

#157975

Purpose

pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

Depositor

Alan Siu Lun Wong

Publication

Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

Type

Empty backbone

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICE HIS3

Plasmid

#122877

Purpose

HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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C121-E12: NanoLuc

Plasmid

#162891

Purpose

Standard Gateway entry clone encoding NanoLuc luciferase

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCre-AntiDsRed

Plasmid

#139674

Purpose

Cassette for testing Cre recombinase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Publication

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Cre recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pL2-G2

Plasmid

#137091

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

GG

Use

Synthetic Biology

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICE MET17

Plasmid

#122880

Purpose

MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pICE LEU2

Plasmid

#122878

Purpose

LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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hsp70:GalT-RFP

Plasmid

#105966

Purpose

heat shock inducible transgenesis, Galactsyl transferase (trans-golgi marker)

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

GalT

Tags

mRFP1

Expression

Bacterial

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAttBFHyg2

Plasmid

#244927

Purpose

attB Hyg Resistant plasmid for RMI targeting an attP site

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2026 Jul 6:iyag166. doi: 10.10

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-URA3

Plasmid

#122907

Purpose

ADE2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 24, 2021

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-URA3

Plasmid

#122915

Purpose

TRP1-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-NatMx

Plasmid

#122918

Purpose

ADE2-NatMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-HphMx

Plasmid

#122916

Purpose

ADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGR-L3S2P00

Plasmid

#46005

Depositor

Christopher Voigt

Publication

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

L3S2P00 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pC-0_v6

Plasmid

#124426

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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