We narrowed to 19,081 results for: cat
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Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
ExpressionYeastPromoterTetO7Available SinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS115 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-Alfa-P2A-TagBFP
Plasmid#199368PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-ALFA-P2A-BFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-ALFA-P2A-TagBFPExpressionMammalianMutationWTAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgRNA1-CTCF-prom
Plasmid#195103PurposeVector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).DepositorInsertsgRNA against CTCF promoter
UseCRISPRExpressionMammalianAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS238D1::sptse5-CT
Plasmid#192959PurposeFor expression of Tse5-CT (1169-1317) in P. putida with the PelB signal peptide fused at the N-terminus in biological function studiesDepositorInsertsptse5-CT
TagsPelB signal peptideExpressionBacterialMutationencodes for tse5 (1169-1317)Available SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgCrebbp#1/Cre
Plasmid#193209PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp geneDepositorAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIAeBlueHPV16LR
Plasmid#185894PurposeIn vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locusDepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2-RacE
Plasmid#188966PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
racE
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER2R
Plasmid#185882PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (11 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRBC
Plasmid#164994PurposeExpression of gRNA targeting TCRbeta constant locusDepositorInsertgRNA against TRBC
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Phage-PGK-FLAG-HA-T(WT)
Plasmid#181737Purposefor lentiviral expression of brachyury ORF in mammalian cellsDepositorInsertT (brachyury)
UseLentiviralMutationa silent mutation in the PAM site of an sgRNAt ta…Available SinceAug. 3, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
Lenti-sgEp300#2/Cre
Plasmid#173592PurposeExpresses a Ep300-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Ep300 (Ep300 Mouse)
UseCRISPR, Cre/Lox, and LentiviralAvailable SinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgTp53#3/Cre
Plasmid#173622PurposeExpresses a Tp53-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Tp53 (Trp53 Mouse)
UseCRISPR, Cre/Lox, and LentiviralAvailable SinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNX19-PX (528-664) (mouse)
Plasmid#119100PurposeBacterial expression of mouse phox homology (PX) domain, SNX19-PX (528-664) (mouse)DepositorAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgPtprd#1/Cre
Plasmid#173647PurposeExpresses a Ptprd-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Ptprd (Ptprd Mouse)
UseCRISPR, Cre/Lox, and LentiviralAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJPF0190
Plasmid#130268PurposePiggybac vector for the expression of mRuby2-fused human PRKACADepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc(W12stop)
Plasmid#180502PurposeMammalian expression of Nluc(W12stop)DepositorInsertNluc(W12stop)
TagsFLAGExpressionMammalianMutationW12(UGG) to amber stop codon(UAG)PromoterCMVAvailable SinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC59-Halo
Plasmid#175327PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_YTHDF1-Halo
Plasmid#175301PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Δ18 B.1.351v2/wtRBD
Plasmid#169467PurposeEncodes SARS-CoV-2 B.1.351v2/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus productionDepositorInsertSARS-CoV-2 B.1.351v2/wtRBD Spike truncated to remove 18 amino acids (S )
ExpressionMammalianAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only