We narrowed to 16,183 results for: nes

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pSpCas9-Puro HLAG-2B-sgRNA

Plasmid

#182552

Purpose

Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

Depositor

Santiago Miriuka

Publication

Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

Insert

hSpCas9-2A-Puro-HLAG-2B-sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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IDG_CLIC2_OE_1

Plasmid

#161642

Purpose

Overexpresses CLIC2

Depositor

Mike McManus

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLIC2 (CLIC2 Human)

Tags

V5

Expression

Mammalian

Available since

Dec. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC59-Halo

Plasmid

#175327

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC59 (CCDC59 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_YTHDF1-Halo

Plasmid

#175301

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

YTHDF1 (YTHDF1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

Plasmid

#170225

Purpose

Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Publication

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

ER (ESR1 Human)

Use

Lentiviral

Tags

V5

Mutation

Y537S

Promoter

EF1a

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh3

Plasmid

#168987

Purpose

Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

Depositor

Ji Luo

Publication

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9 (UBE2I Human)

Expression

Mammalian

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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GP777

Plasmid

#164533

Purpose

Expresses Smo noK-FLAG

Depositor

Gregory Pazour

Publication

Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

Insert

Smoothened noK (Smo Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

All 21 cytoplasmic Lysines of Smoothened changed …

Promoter

CMV

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgGPT2_9

Plasmid

#163456

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

sgRNA 9 targeting GPT2 (GPT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC5-Rap2A-3xFLAG

Plasmid

#163444

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-Rap2A-3xFLAG

Plasmid

#163445

Purpose

lentiviral overexpression vector for human Rap2A

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

Rap2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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NDV-HN-SpyTag

Plasmid

#160000

Purpose

Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

NDV-HN-SpyTag

Tags

His6-tag

Expression

Mammalian

Promoter

Chicken beta-actin promoter

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Publication

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K10-2xCSTREP

Plasmid

#136163

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K10

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K10

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF40-2xCSTREP

Plasmid

#136201

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF40

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF40

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Publication

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Expression

Insect

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Publication

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF46-2xCSTREP

Plasmid

#136207

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF46

Depositor

Britt Glaunsinger

Publication

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF46

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Citrine

Plasmid

#127770

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Publication

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ednrbE2

Use

Enhancer reporter construct

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE1-Citrine

Plasmid

#127773

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer E1 driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Publication

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ednrbE1

Use

Enhancer reporter construct

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-Sox10-FLAG-tev-AVI

Plasmid

#127775

Purpose

Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

Depositor

Tatjana Sauka-Spengler

Publication

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

sox10 (SOX10 Chicken)

Use

Enhancer reporter construct

Tags

Avi

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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