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miniCMV-iRFP670-24xMS2

Plasmid

#238915

Purpose

Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

Depositor

Jian-Hui Jiang

Article

Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

Insert

iRFP670-24xMS2

Use

Lentiviral

Expression

Mammalian

Promoter

miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-tdTomato

Plasmid

#50977

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-tdTomato

Use

AAV

Tags

tdTomato

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

Plasmid

#40132

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease (ELD)

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pRSETa_ mEos4b

Plasmid

#51073

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

Depositor

Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4b

Expression

Bacterial

Promoter

T7

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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SCP2-CMVenh-luc

Plasmid

#22230

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-CMVenh luciferase

Use

Luciferase

Expression

Mammalian

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

Plasmid

#40131

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Tags

FokI nuclease (KKR)

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMC-9-PinII-11

Plasmid

#197769

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PinII terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Frb-N-TEV

Plasmid

#100095

Purpose

SNIPer plasmid, N terminal TEV fused to Frb

Depositor

Jim Wells

Article

Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

Insert

N-TEV

Use

Retroviral

Tags

Frb and Myc

Expression

Mammalian

Mutation

N terminal domain

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HPH

Plasmid

#160216

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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CE-8e-SpRY

Plasmid

#188913

Purpose

A-to-G base editor

Depositor

Xu Ma

Article

Cao et al Mol Ther Nucleic Acids. 2022 May 4;28:73

Insert

nSpRYCas9 ecTadA-8e

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-N-PrLD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_LamB1

Plasmid

#175538

Purpose

Expression of HaloTag9 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-LamB1

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Article

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
RSRF-Luc-2wt

Plasmid

#31818

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Promoter

MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGNW6

Plasmid

#122093

Purpose

suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-oChIEF-citrine

Plasmid

#50974

Purpose

AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

ChIEF gene is mammalian codon optimized

Promoter

human synapsin promoter

Available Since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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HIV-1 integrase F185K/C280S in pET-15b

Plasmid

#61669

Purpose

HIV-1 integrase F185K/C280S in pET-15b

Depositor

Robert Craigie

Article

Jenkins et al J Biol Chem. 1996 Mar 29;271(13):7712-8.

Insert

HIV-1 integrase F185K/C280S

Use

Synthetic Biology

Tags

His

Promoter

T7 promotor

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only