We narrowed to 17,413 results for: puromycin
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Plasmid#190704PurposeExpresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cellsDepositorInsertScrambled sgRNA 2
UseCRISPRExpressionInsectPromoterDrosophila U6Available SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PlmdCas12e KRAB
Plasmid#188506PurposeExpresses FLAG tagged PlmdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-KRAB-NLSExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
∆C' terminal-DPYSL2-pLJC2
Plasmid#189867PurposeExpresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cellsDepositorInsertΔC-DPYSL2
UseLentiviralTags3X FLAGExpressionMammalianMutationdeleted the C'terminus of DPYSL2 (491-572 aa)Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODAL-matMYC
Plasmid#115639PurposeFor targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycyclineDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODALvar-CAG-AAVS1
Plasmid#115262PurposeFor stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cellsDepositorAvailable SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W
Plasmid#163177PurposeLentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tagDepositorAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki-67(RASA)-mCherry_IRESpuro2
Plasmid#182921PurposeExpresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.DepositorInsertMKI67 (MKI67 Human)
TagsEGFP and mCherryExpressionBacterial and MammalianMutationRASA mutationPromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (shuffled Nterm)-mNeonGreen-IRESpuro2
Plasmid#183744PurposeExpresses Ki67 (shuffled Nterm)-mNeonGreenDepositorInsertMKI67 (shuffled Nterm) (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationCD domain with shuffled 4 fragments fused to the …PromoterCMVAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)
Plasmid#189955PurposeshRNA mediated knockdownDepositorInsertERV_MMERVK10c
UseLentiviralAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-PKMYT1-V5-TurboID_IDG-K
Plasmid#135247PurposeGateway destination clone of PKMYT1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertPKMYT1-V5-TurboID (PKMYT1 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianPromoterUbiquitinAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRBC
Plasmid#164994PurposeExpression of gRNA targeting TCRbeta constant locusDepositorInsertgRNA against TRBC
UseCRISPR and LentiviralExpressionMammalianAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_sgEPAS1#1
Plasmid#183119PurposepLentiGuide modified to express GFP and an sgRNa targeting EPAS1DepositorInsertsgEPAS1-1
UseLentiviralPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_GFP_sgEPAS1#2
Plasmid#183120PurposepLentiGuide modified to express GFP and an sgRNA targeting EPAS1DepositorInsertEPAS1-2
UseLentiviralPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide_mCherry_sgEPAS1#2
Plasmid#183123PurposepLentiGuide modified to express mCherry and an sgRNA targeting EPAS1DepositorInsertsgEPAS1-2
UseLentiviralPromoterU6Available SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-H3.3 (H3f3b) sgRNA
Plasmid#186931PurposeH3f3b sgRNA used for genomic targeting at H3f3b C-terminalDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-H3.1 (Hist1h3g) sgRNA
Plasmid#186930PurposeHist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminalDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-H3f3a KO sgRNA
Plasmid#186940PurposeH3f3a KO in mouse ES cellsDepositorAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
px459-H3f3b KO sgRNA
Plasmid#186941PurposeH3f3b KO in mouse ES cellsDepositorAvailable SinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-Tiam1
Plasmid#172102PurposeMammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)DepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mNG-eDHFR(69K6)-p85iSH2
Plasmid#172103PurposeMammalian expression of p85iSH2 fused to mNeonGreen-eDHFR(69K6)DepositorAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only