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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Publication

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Erg

Plasmid

#66977

Purpose

Expression plasmid for mouse Erg; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Erg (Erg Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pBS4S

Plasmid

#55170

Purpose

Empty vector, integration at thrC, ampr, specr

Depositor

Thorsten Mascher

Publication

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Bacillus biobrick box

Promoter

none

Available since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PLKO-mcherry-luc-puro-shSCR

Plasmid

#29780

Depositor

Carl Novina

Publication

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

shSCR

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA-myc-HisA-Sirt3

Plasmid

#24924

Depositor

Toren Finkel

Publication

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMBP-HIS2-Snf7

Plasmid

#21492

Depositor

James Hurley

Publication

Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

Insert

SNF7 (SNF7 Budding Yeast)

Tags

6x His and MBP

Expression

Bacterial

Mutation

none

Available since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pFS181

Plasmid

#12536

Depositor

Nick Rhind

Publication

Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

Insert

human equilibrating nucleoside transporter 1 (SLC29A1 Human)

Expression

Yeast

Available since

Sept. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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OaAEP1_C247A

Plasmid

#200307

Purpose

Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)

Depositor

Artur Krężel

Publication

Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27

Insert

Asparaginyl endopeptidase from Oldenlandia affinis

Tags

Smt3

Expression

Bacterial

Mutation

Cysteine 247 to Alanine

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-LSSmGFP

Plasmid

#186543

Purpose

LSSmGFP fluorescent protein (ex/em = 400/510 nm): cytosolic expression in mammalian cells

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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SGEN

Plasmid

#111171

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Publication

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA-mito-ZapCY1

Plasmid

#58996

Purpose

Mitochondrial Zn-binding FRET biosensor

Depositor

Amy Palmer

Publication

Park et al ACS Chem Biol. 2012 Aug 10.

Insert

mito-ZapCY1 high affinity Zn(II) sensor

Tags

See comment

Expression

Mammalian

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHIS3p:mRuby2-Tub1+3'UTR::HPH

Plasmid

#50633

Depositor

Wei-Lih Lee

Publication

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Lmx1b

Plasmid

#34997

Depositor

Malin Parmar

Publication

Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

Insert

LIM homeobox transcription factor 1-beta (Lmx1b Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUPER IKKalpha

Plasmid

#26208

Depositor

Tom Maniatis

Publication

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK alpha (CHUK Human)

Use

RNAi

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER IKKbeta

Plasmid

#26209

Depositor

Tom Maniatis

Publication

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

pSUPER IKK beta (IKBKB Human)

Use

RNAi

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET His6 StrepII TEV LIC cloning vector (2HR-T)

Plasmid

#29718

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Strep Ii, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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E2F-1 wt-pGex2TK

Plasmid

#21668

Depositor

William Kaelin

Publication

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

E2F-1 (E2F1 Human)

Tags

GST

Expression

Bacterial

Mutation

wild type

Available since

Sept. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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GolgiCKAR

Plasmid

#14871

Depositor

Alexandra Newton

Publication

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

C kinase activity reporter

Tags

CFP, YFP, and eNOS(1-33)

Expression

Mammalian

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMSP1E1D1

Plasmid

#161605

Purpose

Bacterial expression of "extended" membrane scaffold protein (MSP1E1D1) for Nanodisc formation

Depositor

Stephen Sligar

Publication

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E1D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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