We narrowed to 14,795 results for: flag

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pscALPS-HIV-1 Group P-vpr

Plasmid

#115837

Purpose

Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HIV1 Group P Vpr (HQ179987)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-TRAF1

Plasmid

#111199

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

TRAF1 (TRAF1 Human)

Tags

EGFP-FLAG

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE4RA-P2A-Puro

Plasmid

#112672

Purpose

Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE4RA

Use

Lentiviral

Tags

FLAG

Mutation

NLS sequence at the N-terminus and D10A

Promoter

EF1s

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-PGK-Puro

Plasmid

#110860

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Synthetic, S. aureus, Human)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Promoter

U6 promoter;

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-PCYT2

Plasmid

#81074

Purpose

Expresses PCYT2 in mammalian cells

Depositor

Erin O'Shea

Article

Chidley et al Elife. 2016 Jul 12;5. pii: e14601. doi:

Insert

phosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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AAK1

Plasmid

#73244

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

AAK1 (AAK1 Human)

Tags

TEV-His6-Flag

Expression

Bacterial

Mutation

pfam00069:Pkinase 47-307

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pKS011

Plasmid

#65464

Purpose

pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

MBP-Ntag-mCherry

Tags

FLAG and MBP

Expression

Bacterial

Promoter

pl-TetO

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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MSP2372

Plasmid

#73196

Purpose

Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, and Q926A in SpCas9

Promoter

CMV

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PTGFR-Tango

Plasmid

#66487

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

PTGFR (PTGFR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
P2RY10-Tango

Plasmid

#66469

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

P2RY10 (P2RY10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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P2RY14-Tango

Plasmid

#66473

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

P2RY14 (P2RY14 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
HTR1D-Tango

Plasmid

#66406

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

HTR1D (HTR1D Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
HTR1E-Tango

Plasmid

#66407

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

HTR1E (HTR1E Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
LPAR5-Tango

Plasmid

#66421

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

LPAR5 (LPAR5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
LPAR6-Tango

Plasmid

#66422

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

LPAR6 (LPAR6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR63-Tango

Plasmid

#66368

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR63 (GPR63 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR31-Tango

Plasmid

#66351

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR31 (GPR31 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
GPR143-Tango

Plasmid

#66320

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR143 (GPR143 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only