Addgene

Showing: 10221 - 10240 of 24408 results

Results per page:

Sort results by:

pC1-REX-YFP

Plasmid

#48247

Purpose

Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

Depositor

Vsevolod Belousov

Article

Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

Insert

REX-YFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-fDIO-mCherryP2ACre

Plasmid

#161773

Purpose

Flp-Dependent mCherry and Cre expression

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Insert

mCherry-P2A-Cre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXTK066 [Con-Pro_nptII-Broad_PartType8]

Plasmid

#229303

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Constitutive-Promoter_nptII-Broad_PartType8

Depositor

Michael Springer

Article

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

nptII Promoter (Broad)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Fis1

Plasmid

#182580

Purpose

Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

Depositor

Uri Manor

Article

Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

Insert

mCherry-Fis1

Use

Tags

Fis1

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Use

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
NylB'_pET21b

Plasmid

#215416

Purpose

Expression construct for NylB' gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB'

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPANI2A2E2-Crimson

Plasmid

#193758

Purpose

Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PANI2 synthetic promoter carrying binding sites f…

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB_TRE_sg61-PDGFR_EF1a_eGFP

Plasmid

#186310

Purpose

Plasmid for inducible mammalian cell expression of the sg61 helixCAM and constitutive expression of an identifying fluorescent protein (eGFP). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg61-PDGFR
eGFP

Use

Tags

His, myc

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS1NB c Rosella I

Plasmid

#71245

Purpose

a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.

Depositor

Mark Prescott

Article

Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20

Insert

Rosella

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSYN-miRFP670nano3-WPRE-hGHpA

Plasmid

#184688

Purpose

Mammalian expression, miRFP670nano3

Depositor

Axel Nimmerjahn, Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

AAV

Tags

Expression

Mutation

Promoter

hSYN

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Level 1 P2 OsUbiP:Cas9:NosT

Plasmid

#165424

Purpose

GoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassette

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Os Ubiquitin promoter :: Cas9 :: NosTerminator

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-EGFP-eMagA

Plasmid

#162245

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-EGFP-eMagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Plasmid

#219537

Purpose

a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
NLS-tdMCP-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
eMagB-iRFP-Mito

Plasmid

#162250

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagB-iRFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC_MS2_DNA_VLP

Plasmid

#179156

Purpose

Expression of maturation protein and his-tagged coat protein dimer from MS2 phage

Depositor

Paul Freemont

Article

Crone et al GEN Biotechnol. 2022 Dec 1;1(6):496-503.

Inserts

Maturation Protein
Coat Protein Dimer

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Human, Synthetic)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
LT2_12_GlyRNA

Plasmid

#177710

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMYCO1_SpdCas9_pmcDA1

Plasmid

#204615

Purpose

CRISPR cytosine deaminase replicative plasmid for Mycoplasma mycoides subsp. mycoides

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge