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pX335-NEAT1_IS_v2

Plasmid

#97086

Purpose

Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1_IS_v2

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-Rabenosyn-5

Plasmid

#37537

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

Rabenosyn5 (RBSN Human)

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pPLtetO-FtsZ

Plasmid

#89656

Purpose

Plasmid for titrating synthesis of FtsZ with doxycycline

Depositor

Uwe Sauer

Article

Sekar et al Mol Syst Biol. 2018 Nov 5;14(11):e8623.

Insert

GTP-binding tubulin-like cell division protein

Use

Synthetic Biology

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Expression

Bacterial

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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ER2-LSSmGFP

Plasmid

#186538

Purpose

Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Psicheck2 PTPRN2 full length 3'UTR

Plasmid

#26994

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

PTPRN2 Full length 3' UTR (PTPRN2 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pMTIKA

Plasmid

#22061

Depositor

Maria Castro

Article

Xiong et al J Virol. 2006 Jan . 80(1):27-37.

Insert

mCMV-TetOn expression cassette

Expression

Mammalian

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pRK5-Ufm1 WT

Plasmid

#133865

Purpose

Expression of human wild type UFM1

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 (UFM1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CAGhP14

Plasmid

#84362

Purpose

express FLAG-HA tagged human PRDM14

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CAG

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pEGFP-mAV

Plasmid

#129472

Purpose

Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

Depositor

Elizabeth Luna

Article

Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

Insert

Supervillin, isoform 2 (Svil Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET41c-PhTET2

Plasmid

#182428

Purpose

Expresses P. horikoshii tetrahedral aminopeptidase 2 (TET2) in E. coli

Depositor

Bruno Franzetti

Article

Gauto et al Nat Commun. 2022 Apr 8;13(1):1927. doi:

Insert

Tetrahedral aminopeptidase TET2 from P. hirokoshii

Expression

Bacterial

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-C1C2-TS-EYFP

Plasmid

#35520

Depositor

Karl Deisseroth

Article

Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

Insert

ChR1-ChR2 Chimera

Use

Lentiviral

Tags

Trafficking signal and EYFP

Expression

Mammalian

Promoter

CaMKIIa

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE1 - wt)

Plasmid

#16597

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE1 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pKEK3228

Plasmid

#234090

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

aeBlue

Expression

Bacterial

Promoter

pJ23100-BsaI

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ZNF598

Plasmid

#127121

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA ZNF598 (ZNF598 Human)

Use

CRISPR

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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RALA

Plasmid

#55657

Purpose

expression of mouse RALA

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RALA (Rala Mouse)

Tags

GST and Thrombin cleavage site

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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LT2_14_GlyOx

Plasmid

#177711

Purpose

Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)

Depositor

Lisbeth Olsson

Article

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits