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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shNT

Plasmid

#81067

Purpose

Gateway entry vector encoding non-targeting shRNA

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

non-targeting shRNA

Use

Cloning

Tags

Expression

Mutation

Promoter

N/A

Available since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFTK043

Plasmid

#171315

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eGFP fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pre_IV_RFP_1317_v1

Plasmid

#126863

Purpose

1317 b scaffold based on pSB1A3 backbone with RFP gene as insert

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_RFP_1317_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Wnt11 STOP

Plasmid

#35885

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

WNT11 (WNT11 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFTK046

Plasmid

#171318

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

DsRed.T1 fluorescent reporter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proD-B0032-E0051

Plasmid

#107241

Purpose

Expresses lacZa-GFP fusion from constitutive proD promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProA-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proD

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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