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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,118 results for: gibson assembly

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Showing: 1021 - 1040 of 1118 results

  1. pFP.R361

    Plasmid
    #138231
    Purpose
    Expresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Publication
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).BsuP-RIR1*.(CSE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138231-plasmid-map-sequence-id-273489

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  2. pQE80L TriCatcher-MMP

    Plasmid
    #112632
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Publication
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-MMP
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112632-plasmid-map-sequence-id-221417

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  18. pNC-Y

    Plasmid
    #238998
    Purpose
    Node C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZY
    Depositor
    Javier Santos-Moreno
    Publication
    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2
    Insert
    sgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    WT
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    238998-plasmid-map-sequence-id-479360

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Showing: 1021 - 1040 of 1118 results