We narrowed to 19,741 results for: Cre

1. FR_HNF1B_A263insGG

   Plasmid

   #31103

   Depositor

   Gerhart Ryffel

   Article

   Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

   Insert

   HNF1B (HNF1B Human)
   

   Use

   Flp/frt

   Tags

   myc

   Expression

   Mammalian

   Mutation

   mutation A263insGG

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5FRT/TO-UBXD5-Strep-HA

   Plasmid

   #113493

   Purpose

   Expression of human UBXD5 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD5 (UBXN11 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-LmoCas6-KRAB-2a-BlastR

   Plasmid

   #126492

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-KRAB-2a-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCRISPR-v1-sgGPT2_9

   Plasmid

   #163456

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting GPT2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 9 targeting GPT2 (GPT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ntng2.a-Fc-His

   Plasmid

   #72120

   Purpose

   Expresses the extracellular region of the Netrin G2, isoform a protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng2.a (Ntng2 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ntng1.b-Fc-His

   Plasmid

   #72111

   Purpose

   Expresses the extracellular region of the Netrin G1, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntng1.b (Ntng1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sdk1-AP-His

   Plasmid

   #72008

   Purpose

   Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sdk1 (Sdk1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-IgL-C99-T4L-rTA

   Plasmid

   #177840

   Purpose

   Tango assay

   Depositor

   Karsten Melcher

   Article

   Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

   Insert

   C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Flag-EcoCas8e

   Plasmid

   #106270

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasA
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-Flag-EcoCse2

    Plasmid

    #106271

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasB
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-Flag-EcoCas7

    Plasmid

    #106272

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasC
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Flag-EcoCas5

    Plasmid

    #106273

    Purpose

    Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    EcoCasD
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ncam1_1.0.1-Fc-His

    Plasmid

    #72087

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.0.1 Mouse (Ncam1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. P12-bio

    Plasmid

    #47725

    Purpose

    Expresses enzymatically monobiotinylated full-length P12 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P12
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pluc-miR148a-148aT

    Plasmid

    #65053

    Purpose

    Reporter plasmid to detect miR148/152 family expression

    Depositor

    Cristina Fillat

    Article

    Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

    Insert

    Engineered target sites miR-148a-3p (MIR148A Mouse, Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-X1-Neo-DDRGK1-K267R-HA

    Plasmid

    #139848

    Purpose

    Lentiviral expression of human DDRGK1-K267R-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    K267R

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sdk1-Fc-His

    Plasmid

    #72134

    Purpose

    Expresses the extracellular region of the Sdk1 protein (endogenous signal peptide replaced with CD33 signal peptide), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sdk1 (Sdk1 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. BMR1_02g00320-bio-His

    Plasmid

    #108028

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_02g00320
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-uTEV1-MAVS-mTA

    Plasmid

    #170999

    Purpose

    HiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    EGFP-uTEV1-MAVS(tmd)[R537A]
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    MAVS(tmd)[R537A]

    Promoter

    pTRE-tight (TetOn)

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. p3E-DysF

    Plasmid

    #109544

    Purpose

    Multisite gateway vector for 3' tagging with Dysferlin

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Dysferlin (dysf Zebrafish)
    

    Use

    Zebrafish plasmids

    Mutation

    Please see Depositor Comments

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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