We narrowed to 30,783 results for: PLE;

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3TERM_MpNOP1

Plasmid

#136119

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck2

Plasmid

#136127

Purpose

L1 in position 2, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:mT-N7-Ck3

Plasmid

#136128

Purpose

L1 in position 3, control of expression

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:mTurquoise2-N7_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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L1_UBE2:eG-Lt-Ck3

Plasmid

#136129

Purpose

L1 in position 3, mark cell outline with eGFP

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpUBE2:eGFP-Lti6b_3tNos-35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_eGFP-HDEL

Plasmid

#136097

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_eGFP(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CTAG_linker-Lti6b

Plasmid

#136099

Purpose

Plasma Membrane targeting protein for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAla_Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-mVenus

Plasmid

#136101

Purpose

FP for C-term fusion with CDS12 without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSerGly10xAlaGlySer_mVenus

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_linker-N7

Plasmid

#136102

Purpose

Nuclear targeting peptide for C-term fusion with CDS12 without linker.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_AlaSer4xAla_N7

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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5UTR_MpNOP1

Plasmid

#136103

Purpose

5UTR for cloning with PROM_MpNOP1

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpNOP1 Mapoly0008s0036

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mTurquoise2-HDEL

Plasmid

#136105

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mTurquoise2(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_mVenus-HDEL

Plasmid

#136107

Purpose

FP with ER retention signal for C-term fusion with CDS12 with ER targeting signal peptide

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_mVenus(noATG,noStop)-HDEL

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CTAG_NLS-SV40

Plasmid

#136108

Purpose

CTAG Nuclear localiation signal SV40 for fusion with CDS12

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CTAG_NLS-SV40

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB_spacer

Plasmid

#136076

Purpose

Use when making odd level constructs (L3,...) if pCsB position (position 2) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mTurquoise2-linker

Plasmid

#136090

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mTurquoise2(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_mVenus-linker

Plasmid

#136092

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mVenus(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CDS12_TP-MpSIG2

Plasmid

#136093

Purpose

Chloroplast transit peptide from MpSIG2. For N-term fusion with a CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

TP-MpSIG2, Mapoly0214s0004

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsA_spacer

Plasmid

#136075

Purpose

Use when making odd level constructs (L3,...) if pCsA position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH580

Plasmid

#172164

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2v2+B); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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