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DCT.VV.Orf263

Plasmid

#12951

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf263

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
ZP61 Isthmin

Plasmid

#16881

Depositor

Randall Moon

Article

Weidinger et al Curr Biol. 2005 Mar 29. 15(6):489-500.

Insert

isthmin (ism1 Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-SpEAF 60-251 pBacPAK8

Plasmid

#15321

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

EAF1 (eaf1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Mutation

N terminal deletion lacking first 59 amino acids …

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf82

Plasmid

#12770

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf82

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pBS Calponin3

Plasmid

#19194

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Calponin 3 (CNN3 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf152

Plasmid

#12840

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf152

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pLX304-Lantern-MAVS

Plasmid

#250917

Purpose

Expresses Lantern fused to the C-terminal 31 amino acids of MAVS in mammalian cells for targeting to the outer mitochondrial membrane

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

Lantern

Use

Lentiviral

Tags

MAVS C-terminal fragment and V5

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:100_offset

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmB-HNRNPL_sgRNA_3

Plasmid

#242892

Purpose

PiggyBac cargo vector with HNRNPL sgRNA 3 for dox-inducible knockdown

Depositor

Mauro Calabrese

Article

Murvin et al bioRxiv 2025.07.16.665154

Insert

HNRNPL (Hnrnpl Mouse)

Use

CRISPR

Promoter

U6

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLX304-mito-Lantern

Plasmid

#250920

Purpose

Expresses Lantern fused to the N-terminal 23 amino acid residues of human COX4 in mammalian cells

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

mito-Lantern

Use

Lentiviral

Tags

COX4 mitochondrial targeting sequence, V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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E. coli Beta sliding clamp (dnaN) in pet28PP

Plasmid

#217412

Purpose

Expresses E. coli Beta sliding clamp with a His-tag in bacterial cells

Depositor

Brian Kelch

Article

Landeck et al J Biol Chem. 2024 Apr;300(4):107166. doi

Insert

Beta sliding clamp (dnaN E. coli)

Tags

His

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-nCas9-PolI5M

Plasmid

#249066

Purpose

Expresses nucleus-localized nCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

nCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEvolvR-mCherry-enCas9(D10A)-PolI5M

Plasmid

#249060

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-dCas9-PolI5M

Plasmid

#249065

Purpose

Expresses nucleus-localized dCas9 fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

dCas9-PolI5M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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p-mCherry-enCas9(D10A)

Plasmid

#249063

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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huADF S3A

Plasmid

#245943

Purpose

Expressing a constitutively active human ADF

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Mutation

S3A

Promoter

CMV

Available Since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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