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pLNHA-EGFP-C1-HsNot1iso1_1595-2376_S

Plasmid

#147491

Purpose

Mammalian Expression of HsNot1iso1_1595-2376

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso1_1595-2376 (CNOT1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del380-404-W507E_S

Plasmid

#147499

Purpose

Mammalian Expression of HsNot2-del380-404-W507E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-del380-404-W507E (CNOT2 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G

Plasmid

#146408

Purpose

Bacterial Expression of HsDCP1a_539-582-mut1

Depositor

Elisa Izaurralde

Publication

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut1 (DCP1A Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_G

Plasmid

#146410

Purpose

Bacterial Expression of HsDCP1a_539-582-mut3

Depositor

Elisa Izaurralde

Publication

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a_539-582-mut3 (DCP1A Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-del155-168_G

Plasmid

#146413

Purpose

Mammalian Expression of HsDcp1a-del155-168

Depositor

Elisa Izaurralde

Publication

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-del155-168 (DCP1A Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-del539-582_G

Plasmid

#146414

Purpose

Mammalian Expression of HsDcp1a-del539-582

Depositor

Elisa Izaurralde

Publication

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a-del539-582 (DCP1A Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-tubcDNA_D

Plasmid

#146140

Purpose

Mammalian Expression of tubcDNA

Depositor

Elisa Izaurralde

Publication

Wittkopp et al Mol Cell Biol. 2009 Jul . 29(13):3517-28

Insert

tubcDNA

Expression

Mammalian

Mutation

5 silent and one non silent mutation E442K compar…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-NHERF1 PDZ1/2*

Plasmid

#195211

Purpose

Mammalian expression vector for GFP tagged NHERF1. Dual PDZ1/2 GYGF>GYAA motif mutant

Depositor

Anthony Bretscher, David Bryant

Publication

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Mutation

Dual PDZ1/2 GYGF>GYAA motif mutant

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-nCLC

Plasmid

#193564

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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nCLC-ShadowY

Plasmid

#193565

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

ShadowY

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-CLC

Plasmid

#193567

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CLC-FKBP-EGFP

Plasmid

#193572

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

FKBP and mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Tags

ShadowY and mEGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-CLCdeltaN

Plasmid

#193562

Purpose

Expresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Publication

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Mutation

Deleted amino acids 1-89

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PKC1-2GFP-ter-TRP1

Plasmid

#184773

Purpose

Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

PKC1 (PKC1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-HsGIGYF1_1-671-del286-316_AI

Plasmid

#148959

Purpose

Mammalian Expression of HsGIGYF1_1-671-del286-316

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671-del286-316 (GIGYF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsGIGYF1_1-671-del286-316_AI

Plasmid

#148963

Purpose

Mammalian Expression of HsGIGYF1_1-671-del286-316

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF1_1-671-del286-316 (GIGYF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRck-C313AL317A_AI

Plasmid

#148965

Purpose

Mammalian Expression of HsRck-C313AL317A

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsRck-C313AL317A (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsRck-CLLKtoAAAA_AI

Plasmid

#148966

Purpose

Mammalian Expression of HsRck-CLLKtoAAAA

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsRck-CLLKtoAAAA (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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