We narrowed to 14,430 results for: NTS

1. EBFP2-Cx43

   Plasmid

   #69020

   Purpose

   Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Tags

   EBFP2

   Promoter

   CMV

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. HP21-TPI-PTC1

   Plasmid

   #131043

   Purpose

   Expresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTR

   Depositor

   Marvin Tanenbaum

   Article

   Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

   Insert

   TPI-PTC1 (TPI1 Human)
   

   Expression

   Mammalian

   Mutation

   PTC1

   Promoter

   Pcmv

   Available Since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX-TRV2-vCHLI

   Plasmid

   #239842

   Purpose

   Derivative of pLX-TRV2, a tobacco rattle virus (TRV) vector, designed for the expression of a 32-nt insert to silence CHLI homolog genes in Nicotiana benthamiana and Solanum aethiopicum

   Depositor

   Fabio Pasin

   Article

   Garcia et al Plant Biotechnol J. 2025 Nov;23(11):4930

   Insert

   vCHLI
   

   Use

   RNAi

   Expression

   Plant

   Available Since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDC32B-B/c

   Plasmid

   #227963

   Purpose

   Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPEY-Z3-Nat-ADE2

   Plasmid

   #232106

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPEY-Z3-Hyg-ADE2

   Plasmid

   #232107

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

   Plasmid

   #221994

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   TALE: Q231R, W232G, S233A | DddA: T1413I

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-601[insert(+1) TA-rich]

   Plasmid

   #221196

   Purpose

   601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601

   Depositor

   Gregory Bowman

   Article

   Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

   Insert

   601[insert(+1)TA-rich]
   

   Use

   Unspecified

   Mutation

   601 has an additional mutation added 22 bp from d…

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUPD2 linker-RDF (GB2892)

    Plasmid

    #160604

    Purpose

    RDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequence

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    RDF
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0.178

    Plasmid

    #119645

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS3 Down Flank
    

    Use

    Synthetic Biology

    Mutation

    4 nucleotide insertion between nt 65 and 66 in NS…

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0.165

    Plasmid

    #119634

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    6803 NS1 Up Flank
    

    Use

    Synthetic Biology

    Mutation

    1 nucleotide insertion between nt 21 and 22 in NS…

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0.175

    Plasmid

    #119643

    Purpose

    Level 0 Part. Insertion site

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Insert

    7942 NS2 Up Flank
    

    Use

    Synthetic Biology

    Mutation

    bp 422 A to T, bp 423 A to T, bp 663 inserted G. …

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVR2

    Plasmid

    #84719

    Purpose

    E. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcription

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Expression

    Bacterial

    Mutation

    12 nt stretch lacking A's inserted before 5&…

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS2002

    Plasmid

    #239832

    Purpose

    Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    NovaIscB omegaRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Replacement of A12-A57 with GAAA in omegaRNA scaf…

    Promoter

    human U6

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-BLM

    Plasmid

    #80070

    Purpose

    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

    Depositor

    Nathan Ellis

    Article

    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

    Insert

    BLM (BLM Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    If correct ID, the cDNA is from HeLa and has char…

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACPPiso1-V5

    Plasmid

    #113052

    Purpose

    Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 1 (ACP3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACPPiso2-V5

    Plasmid

    #113053

    Purpose

    Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

    Insert

    prostatic acid phosphatase isoform 2 (ACP3 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)

    Plasmid

    #221998

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    TALE: Q231R, W232G, S233A | DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MBP-MEK1 S222TAG

    Plasmid

    #68302

    Purpose

    N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    MBP-MEK1 S222TAG (MAP2K1 Synthetic, Human)
    

    Tags

    His and MBP

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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