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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Plasmid

#187077

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in the mitochondria matrix

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_Cox8-HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Plasmid

#187078

Purpose

Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in lysosomes

Depositor

Kai Johnsson

Article

Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

Insert

TOM20-dHaloTag7_T2A_LamP1- HaloTag7

Expression

Mammalian

Mutation

dHaloTag7 = HaloTag7-D106A

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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GB4192

Plasmid

#215250

Purpose

TU for the expression of the CRY2(PHR) gene fused to the Etr binding domain under the P35S promoter and TNOS terminator in alpha1.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:EtrBD:CRY2PHR:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSJX067

Plasmid

#232328

Purpose

Genome editing in Agrobacterium fabrum

Depositor

Wei Zhao

Article

Sun et al Nucleic Acids Res. 2025 Apr 22;53(8):gka

Inserts

sgRNA
SpCas9M

Use

CRISPR

Tags

H-arms and sfGFP

Expression

Bacterial

Promoter

PJ23119 and Pvan

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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IMPT-11371

Plasmid

#236191

Purpose

Express GPR119 in insect cells

Depositor

Vadim Cherezov

Article

Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

Insert

GPR119 (GPR119 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmF

Plasmid

#226912

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in forward orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPSmR

Plasmid

#226913

Purpose

Minimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientation

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2025 Jun 4;37(6):koaf084. do

Type

Empty backbone

Expression

Plant

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_4_034_aadA

Plasmid

#235903

Purpose

Aminoglycoside-3''-9-adenylyltransferase selection gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

aadA

Use

Synthetic Biology

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-W

Plasmid

#238064

Purpose

Expresses rat CENP-W; untagged; made for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Rat CENP-W (Cenpw Rat)

Expression

Mammalian

Promoter

T7

Available Since

May 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha TDH

Plasmid

#236457

Purpose

stable expression of TDH using viral infection

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

TDH (Tdh Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG p85beta

Plasmid

#237336

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

p85beta (PIK3R2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pYihI-C-term K-R

Plasmid

#233092

Purpose

Expression of GST-YihI with C-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Article

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

GST-YihI with C-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

C-term lysines (K) mutated to arginine (R)

Available Since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pME_Cp_0_4_024_CrCD-marker

Plasmid

#235900

Purpose

Cytosine deaminase selection gene, codon optimized for Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

CrCD-marker

Use

Synthetic Biology

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_061_gene10_5'UTR_T7

Plasmid

#235852

Purpose

gene10 5'UTR T7

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

gene10 5'UTR T7

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3a_001_gene10_5'UTR_T7

Plasmid

#235854

Purpose

gene10 5'UTR T7 3a

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

gene10 5'UTR T7

Use

Synthetic Biology

Mutation

none

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mCardinal

Plasmid

#228630

Purpose

Mammalian expression of mCardinal and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mPapaya

Plasmid

#228626

Purpose

Mammalian expression of mPapaya and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RFP-SIKE

Plasmid

#235691

Purpose

Express RFP tagged SIKE gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

SIKE (SIKE1 Human)

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKEK3220

Plasmid

#234099

Purpose

CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

Depositor

Karl Klose

Article

Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

Insert

sfGFP

Tags

FLAG

Expression

Bacterial

Mutation

S30R/Y39N/N105T/Y145F/I171V/A206V

Promoter

pJ23100-BsaI

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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