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pHK302.1

Plasmid

#235466

Purpose

Message phagemid carrying sgRNA1 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA1

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.2

Plasmid

#235467

Purpose

Message phagemid carrying sgRNA2 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA2

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.3

Plasmid

#235468

Purpose

Message phagemid carrying sgRNA3 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA3

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.4

Plasmid

#235469

Purpose

Message phagemid carrying sgRNA4 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA4

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHK302.5

Plasmid

#235470

Purpose

Message phagemid carrying sgRNA5 (prom. J23110, backbone pBR322)

Depositor

Yolanda Schaerli

Publication

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

sgRNA5

Use

Synthetic Biology

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mStayGold-Hyg

Plasmid

#236486

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mStayGold and selection on Hyg.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mStayGold

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mCherry-Nat

Plasmid

#236488

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mCherry and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mCherry

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Hyg

Plasmid

#236480

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Hyg.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mScarlet-Nat

Plasmid

#236491

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mScarlet

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Nat

Plasmid

#236476

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

GFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Publication

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

mNeonGreen

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGG-PIP72

Plasmid

#196810

Purpose

Contains PMR4 promoter

Depositor

Marc Somssich

Publication

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

POWDERY MILDEW RESISTANT 4

Use

Greengate (gg) entry vector

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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P30.pA.NGFR-mSrtA.mCMV.hPGK.GFP.wpre

Plasmid

#226728

Purpose

backbone vector, GFP expression

Depositor

Ferdinando Pucci

Publication

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Type

Empty backbone

Expression

Mammalian

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-10 kb

Plasmid

#218318

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Expression

Mammalian

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAM305

Plasmid

#226137

Purpose

Expression of His6-mEos3.2-UbK48R

Depositor

Andreas Martin

Publication

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-mEos3.2-UbK48R

Expression

Bacterial

Mutation

K48R

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-FL_WobbleMut

Plasmid

#209053

Purpose

Gateway-compatible entry vector, with insert of the full length TAF5 gene mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Publication

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 full length

Use

Gateway Cloning

Expression

Mammalian

Available since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pITESceIcmd

Plasmid

#218288

Purpose

Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

Depositor

Claudia Vickers

Publication

McDonnell et al bioRxiv 2024.03.26.586389

Insert

HO(-253, -1)-tSynth3

Expression

Yeast

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo7

Plasmid

#203958

Purpose

Cloning plasmid containing the ampicillin resistance gene insert

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Ampicillin Resistance Gene

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo3

Plasmid

#203921

Purpose

Cloning plasmid containing the ColE1 origin insert with a chloramphenicol resistance marker

Depositor

Nathan Crook

Publication

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

ColE1 Origin of Replication

Expression

Plant

Promoter

N/A

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-dMASS_1_WPRE

Plasmid

#209765

Purpose

AAV virus production for neuronal expression of dMASS_1 under hSyn promoter

Depositor

Andre Berndt

Publication

Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

Insert

dMASS_1

Use

AAV

Expression

Mammalian

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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