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pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pET23a-MmRPS27a

Plasmid

#69559

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Mouse, Human)

Expression

Bacterial

Mutation

Changed Alanine 133 (human RPS27a) to Glycine (mo…

Promoter

T7

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28b-Sad1

Plasmid

#90297

Purpose

protein expression

Depositor

Christine Guthrie

Article

Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

Insert

SAD1

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a - CPX

Plasmid

#89620

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi cytoplasmic tryparedoxin peroxidase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Cytosolic Tryparedoxin peroxidase

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-API5

Plasmid

#157655

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

His tag

Expression

Bacterial

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-HpDsbG

Plasmid

#39329

Depositor

SeWon Suh

Article

Yoon et al FEBS Lett. 2011 Dec 15;585(24):3862-7. E

Insert

oxidoreductase

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
pET28a Gx-NL2

Plasmid

#169107

Purpose

Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Article

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-BCA-P(11)4

Plasmid

#185780

Purpose

Encodes enzyme bovine carbonic anhydrase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

BCA-P(11)4

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a - SodB

Plasmid

#89613

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase B protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase B

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

April 20, 2017

Availability

Academic Institutions and Nonprofits only

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