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pYPQ-Cas9-Act3.0

Plasmid

#178954

Purpose

It consists of a catalytically active Cas9 nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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Activin A (S2-2)

Plasmid

#184621

Purpose

for transfection into S2 cells and expression of Activin A protein

Depositor

Timothy Springer

Article

Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

Insert

INHBA (INHBA Human)

Tags

8x His - streptavidin binding peptide

Expression

Insect

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-SpRY-Act3.0

Plasmid

#178957

Purpose

It consists of a catalytically active SpRY nuclease and MS2-SunTag-activators activation complex (ScFv-sfGFP-2xTAD), enabling simultaneous genome editing and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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JF001A

Plasmid

#53116

Purpose

expresses alpha hemolysin under the control of theophylline riboswitch

Depositor

Sheref Mansy

Article

Lentini et al Nat Commun. 2014 May 30;5:4012. doi: 10.

Insert

theophylline riboswitch and alpha hemolysin

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pLC-EGFP-RIP1

Plasmid

#75163

Purpose

LentiCRISPR-EGFP with sgRNA targeting human RIPk1

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

RIPK1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pFAB3677

Plasmid

#47833

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 2 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3737

Plasmid

#47838

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 7 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3689

Plasmid

#47834

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 3 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3893

Plasmid

#47844

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 13 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3725

Plasmid

#47837

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 6 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3869

Plasmid

#47842

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 11 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3992

Plasmid

#47823

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD16

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1935

Plasmid

#47860

Purpose

BIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

his_T_min_linker_crp_T_min double terminator

Use

Synthetic Biology

Expression

Bacterial

Mutation

concatenate of his_min and crp_min

Promoter

pLTetO15

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3913

Plasmid

#47816

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD9

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3665

Plasmid

#47832

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 1 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 1 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3701

Plasmid

#47835

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 4 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 4 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3845

Plasmid

#47840

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 9 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 9 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3857

Plasmid

#47841

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 10 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 10 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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