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pCMV_eA3A T31A-NG-nCas9

Plasmid

#163539

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pC-0

Plasmid

#124421

Purpose

Full MCS (step 2)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNMHCII-C1-GFP

Plasmid

#46041

Depositor

Robert Adelstein

Publication

Jana et al J Biol Chem. 2006 Aug 25;281(34):24662-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pRRL TGFΒR2-mEmerald

Plasmid

#258443

Purpose

Lentiviral expression of mEmerald-tagged TGF-ΒR2

Depositor

Matthew Kutys

Publication

Xia et al Adv Sci (Weinh). 2026 Jun 17:e10448. doi

Insert

TGFBR2 (TGFBR2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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shortVGLL3-GFP

Plasmid

#241148

Purpose

expresses short isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

shortVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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longVGLL3-GFP

Plasmid

#241149

Purpose

expresses long isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

longVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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Smarce1-Ccnb1-mitotic degron [R42A mutant] donor template (Flox-PGK-NeoR)

Plasmid

#259650

Purpose

Homologous recombination donor template to generate Smarce1-Ccnb1 mitotic degron [R42A mutant] knockin mouse embryonic stem cells

Depositor

Charles Roberts

Publication

Zhu et al Nature. 2023 Jun;618(7963):180-187. doi:

Insert

Smarce1 (Smarce1 Mouse)

Use

Mouse Targeting

Mutation

R42A mutation in Ccnb1

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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LA-ELP-ybbR-RLC-ddfln4-Xmod-DocIII-Ctag

Plasmid

#257335

Purpose

Polyprotein for applying force to the N- and C-termini of the RLC in the RLC complex of human β-cardiac myosin

Depositor

Tom Perkins

Publication

Deopa et al ACS Nano. 2026 Jun 9;20(22):15982-16000.

Inserts

RLC (MYL2 Human)
Lever Arm (MYH7 Human)

Tags

Ctag, Dockerin III, ELP120 (Elastin-Like Polypept…

Expression

Mammalian

Mutation

only amino acids # 810-846 are used

Promoter

T7

Available since

July 17, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_4ED3_C5K

Plasmid

#251255

Purpose

To express the dengue virus serotype 3 envelope protein domain III with a five-lysine tag at the C-terminus in E. coli

Depositor

Yutaka Kuroda

Publication

Rahman et al Front Immunol. 2021 Jun 11;12:671590. do

Insert

DENV_4ED3

Tags

5xLys with a GG linker and 6xHis, 11 aa linker, t…

Expression

Bacterial

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_4ED3_C5D

Plasmid

#251254

Purpose

To express the dengue virus serotype 3 envelope protein domain III with a five-aspartate tag at the C-terminus in E. coli

Depositor

Yutaka Kuroda

Publication

Rahman et al Front Immunol. 2021 Jun 11;12:671590. do

Insert

DENV_4ED3

Tags

5xAsp with a GG linker and 6xHis, 11 aa linker, t…

Expression

Bacterial

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_3ED3_C5K

Plasmid

#251253

Purpose

To express the dengue virus serotype 3 envelope protein domain III with a five-lysine tag at the C-terminus in E. coli

Depositor

Yutaka Kuroda

Publication

Rahman et al Front Immunol. 2021 Jun 11;12:671590. do

Insert

DENV_3ED3

Tags

5xLys with a GG linker and 6xHis, 11 aa linker, t…

Expression

Bacterial

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_3ED3_C5D

Plasmid

#251252

Purpose

To express the dengue virus serotype 3 envelope protein domain III with a five-aspartate tag at the C-terminus in E. coli

Depositor

Yutaka Kuroda

Publication

Rahman et al Front Immunol. 2021 Jun 11;12:671590. do

Insert

DENV_3ED3

Tags

5xAsp with a GG linker and 6xHis, 11 aa linker, t…

Expression

Bacterial

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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DENV_3ED3

Plasmid

#251248

Purpose

To express the dengue virus serotype 3 envelope protein domain III in E. coli

Depositor

Yutaka Kuroda

Publication

Elahi et al Proteins. 2013 Jun;81(6):1090-5. doi: 10

Insert

DENV_3ED3

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available since

June 4, 2026

Availability

Academic Institutions and Nonprofits only

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TEVEp_pRK793

Plasmid

#247672

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVEp insert based on Yi et al., 2013.

Depositor

Carl Denard

Publication

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVEp

Tags

His and MBP

Expression

Bacterial

Mutation

S120R, D148R, T173A, N177K, M218I, S219P

Available since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-PHO8 K(24,25,30,33)R

Plasmid

#207044

Purpose

Expression of GFP-Pho8 point mutant under CUP1 promoter.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

GFP

Expression

Yeast

Mutation

K(24,25,30,33)R

Promoter

pCUP1

Available since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

Plasmid

#241143

Purpose

Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent SunTag-tagged proteins and track MS2 and PP7-tagged mRNA

Depositor

Tim Stasevich

Publication

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-GCN4 scFv
tdMCP
tdPCP

Tags

(n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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ADH1pro_LEU3_SIR4

Plasmid

#190582

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Publication

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LEU3 (LEU3 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LYS14_SIR4

Plasmid

#190583

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Publication

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LYS14 (LYS14 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_YRR1_SIR4

Plasmid

#190585

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Publication

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

YRR1 (YRR1 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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