We narrowed to 26,402 results for: Vit

1. pCRIS-PITChv2-dTAG-BSD (BRD4)

   Plasmid

   #91795

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-BSD knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRIS-PITChv2-BSD-dTAG (BRD4)

   Plasmid

   #91792

   Purpose

   PITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   BSD-P2A-2xHA-FKBP_F36V
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promoterless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-N-Flag-Caspase-1

   Plasmid

   #75128

   Purpose

   Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   caspase 1 (Casp1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P341S in Caspase-1 (please see depositor comments…

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. (attB) tdMCP-tagged BRaf with MS2-circRNA

   Plasmid

   #226163

   Purpose

   Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   3xFlag-tdMCP-BRaf (BRAF Human)
   IRES-mCherry-P2A-PuroR
   MS2-circRNA barcode
   

   Use

   Bxb1 attb, no promoter

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Available Since

   Aug. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. GABBR1-DuET

   Plasmid

   #213247

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GABBR1 (GABBR1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

   Plasmid

   #83842

   Purpose

   Fluorescent probe for S/G2 cell cycle transition and G2/M transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CXCR7-DuET

   Plasmid

   #213224

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CXCR7 (ACKR3 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLAG-HA-FbxO11-ΔFbox-pcDNA3.1-

   Plasmid

   #52502

   Purpose

   expresses human FbxO11-ΔFbox in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   deletion of Fbox (deleted aa 71-116)

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. LPAR4-DuET

   Plasmid

   #213331

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   LPAR4 (LPAR4 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-puro/Flag PD-L1 WT

    Plasmid

    #121446

    Purpose

    expression of huamn PD-L1 wildtype in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    wildtype

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas12Max-ABE

    Plasmid

    #195335

    Purpose

    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-ABE
    

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-CMV-jGCaMP7f

    Plasmid

    #104483

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7f
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CDK2 gRNA (BRDN0001148950)

    Plasmid

    #77193

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK2 (CDK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-CMV-hXRCC1-mCherry-Hygro

    Plasmid

    #176532

    Purpose

    mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

    Insert

    X-Ray Repair Cross Complementing 1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORTMAGE311B

    Plasmid

    #120418

    Purpose

    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

    Depositor

    Csaba Pál

    Article

    Szili et al bioRxiv (2018)

    Inserts

    MutL E32K
    Lambda Beta
    XylS
    

    Expression

    Bacterial

    Mutation

    E32K mutation conferring dominant mutator phenoty…

    Promoter

    Pm

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-Neo/Myc PD-L1

    Plasmid

    #121477

    Purpose

    expression of human Myc-PD-L1 in mamalian cell

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. hfCas12Max

    Plasmid

    #195336

    Purpose

    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized hfCas12Max
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    N243R, E336R, D892R

    Promoter

    CBh, CMV, hU6

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-SFFV-mtagBFP-2A STING V155M

    Plasmid

    #102591

    Purpose

    Lentivector to express STING V155M and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTT5-hSDC1

    Plasmid

    #52326

    Purpose

    expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Expression

    Mammalian

    Mutation

    R117Q mutation (R95Q according to numbering in Fi…

    Promoter

    CMV

    Available Since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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