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pPTK002-2-pGAP

Plasmid

#84961

Purpose

Type 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Glyceraldehyde-3-phosphate dehydrogenase promoter

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1805 c to g)

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFTK020

Plasmid

#171292

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PalcA syn_NoCrea (ANIA_08979) promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK004

Plasmid

#171276

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgpdA ANIA_08041 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-U6a

Plasmid

#107593

Purpose

An entry vector with U6a promoter driving guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

U6a promoter

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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p221z-AtMIR390a

Plasmid

#118388

Purpose

Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

AtMIR390a backbone

Use

RNAi

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSW549 YFP/mVenus

Plasmid

#115984

Purpose

entry vector for GreenGate cloning method, CDS

Depositor

Jan Lohmann

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

mVenus

Use

Unspecified

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEN_TmiRc3_shURB5

Plasmid

#81066

Purpose

Gateway entry vector encoding shRNA to UBR5

Depositor

Darren Saunders

Article

Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

Insert

shRNA targeting UBR5

Use

Cloning

Promoter

N/A

Available Since

Jan. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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