We narrowed to 26,403 results for: Vit

1. pAAV-P3-Alb-mNG

   Plasmid

   #183460

   Purpose

   To produce AAV with the P3 promoter to express Alb(mouse)-mNeonGreen fusion protein

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   Alb (Alb Mouse)
   

   Use

   AAV

   Tags

   mNeonGreen

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. F2R-DuET

   Plasmid

   #213234

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   F2R (F2R Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FSHR-DuET

   Plasmid

   #213242

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   FSHR (FSHR Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRIG-Akt1

   Plasmid

   #53583

   Purpose

   lentiviral expression of constitutively active human Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Oct. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3xFLAG CMV14 / BRAF-G469V

   Plasmid

   #131718

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G469V

   Promoter

   CMV

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-MAPK14

   Plasmid

   #200973

   Purpose

   a mammalian expression vector to express MAPK14

   Depositor

   Jason Sheltzer

   Article

   Bhattacharjee et al Cell Chem Biol. 2023 Oct 19;30(10):1211-

   Insert

   mitogen-activated protein kinase 14 (MAPK14) (MAPK14 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-hygro-STING V155M

   Plasmid

   #102599

   Purpose

   Express STING V155M for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   V155M

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMega-MaPylRS

   Plasmid

   #200225

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   proK-lacO and tacI

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-terminal 3X Flag Human CASP9 C287A Mutant

   Plasmid

   #198380

   Purpose

   Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
   

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Cysteine 287 to Alanine to eliminate prot…

   Promoter

   CMV

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBA950

    Plasmid

    #122239

    Purpose

    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.

    Depositor

    Jonathan Weissman

    Article

    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

    Insert

    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CCR2-DuET

    Plasmid

    #213198

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CCR2 (CCR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-hygro-STING HAQ

    Plasmid

    #102600

    Purpose

    Express STING HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R71H-G230A-R293Q (HAQ)

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-AcGFP-Sec61-IRES-Blast

    Plasmid

    #154009

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    acGFP-Sec61 (SEC61B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIP-SFFV-mtagBFP-2A STING-342STOP

    Plasmid

    #102588

    Purpose

    Lentivector to express STING delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    delta 342

    Promoter

    SFFV

    Available Since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CXCR3-DuET

    Plasmid

    #213220

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CXCR3 (CXCR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-C2m2-mKate

    Plasmid

    #208793

    Purpose

    For AAV production to express C2m2-mKate in astrocytes

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Use

    AAV

    Tags

    mKate

    Expression

    Mammalian

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    GFAP promoter

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-SARS2-S-D614 (C-flag)

    Plasmid

    #156420

    Purpose

    Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

    Depositor

    Hyeryun Choe, Michael Farzan

    Article

    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

    Insert

    SARS-CoV-2 S protein (S SARS CoV2)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CNR1-DuET

    Plasmid

    #213213

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    CNR1 (CNR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-gfap

    Plasmid

    #75024

    Purpose

    Can be used to drive expression in zebrafish astrocytes.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    glial fibrillary acidic protein promoter (gfap Zebrafish)
    

    Use

    5' entry vector for multisite gateway three …

    Promoter

    gfap

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniCRISPRoff-v1

    Plasmid

    #197030

    Purpose

    Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized miniCRISRPoff-v1
    

    Expression

    Mammalian

    Mutation

    D52R / T132R / D228A / D406A

    Promoter

    CBh, CMV, hU6

    Available Since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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