We narrowed to 15,772 results for: LEA

1. csr-1_son-1_iRFP670

   Plasmid

   #191749

   Purpose

   Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Insert

   NCU04288 (NCU04288 Neurospora crassa)
   

   Tags

   NCU04502 promoter and iRFP670

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEXC3GH (Kan)

   Plasmid

   #110081

   Purpose

   Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PsaA

   Plasmid

   #159997

   Purpose

   Expresses Streptococcus pneumoniae PsaA (19-309) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   PsaA (19-309)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA-FRT-FAK20

   Plasmid

   #59120

   Purpose

   FAK fret sensor with 20 nm helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2-Cer.zf1

   Plasmid

   #61389

   Purpose

   mRNA synthesis of cerulean codon modified for zebrafish

   Depositor

   Harold Burgess

   Article

   Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

   Insert

   Cer.zf1
   

   Use

   Zebrafish expression

   Promoter

   sp6

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA_crE6G2

   Plasmid

   #172929

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6G2
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Val317Gly, Met316Gly

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Spy0469

   Plasmid

   #159996

   Purpose

   Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   Spy0469 (24-389)
   

   Tags

   His6-tag and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS1-rab-3P::his-24::tagBFP

   Plasmid

   #124343

   Purpose

   Nuclear Localized Pan-Neuronal tagBFP

   Depositor

   Andrew Leifer

   Article

   Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

   Insert

   rab-3, tagBFP (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA-FRT-FAK0

   Plasmid

   #45047

   Purpose

   FAK fret sensor without helix

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

   Insert

   Focal Adhesion Kinase
   

   Tags

   FLAG, mCerulean, and mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PH-dL5**

    Plasmid

    #96946

    Purpose

    PLC pleckstrin homology domain linked to FAP for CALI validation

    Depositor

    Marcel Bruchez

    Article

    He et al Nat Methods. 2016 Mar;13(3):263-268. doi

    Insert

    Pleckstrin homology domain
    

    Tags

    dL5 and mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. NLS-FUS-Citrine-PixE

    Plasmid

    #111506

    Purpose

    For the nuclear localization of the PixELL system

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    PixE (slr1693 )
    

    Use

    Lentiviral

    Tags

    Citrine, FUS, and NLS

    Promoter

    SFFV

    Available Since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos3.2-LaminA-C-18

    Plasmid

    #57463

    Purpose

    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Insert

    LaminA (LMNA Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pICSL50015

    Plasmid

    #50318

    Purpose

    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, mNEON (Branchistoma lanceolatum)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNUT3

    Plasmid

    #175744

    Purpose

    Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

    Depositor

    Yubin Zhou

    Article

    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

    Insert

    mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CHD1 (CHD1A-c107)

    Plasmid

    #110253

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CHD1 (CHD1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME nls-eos

    Plasmid

    #73791

    Purpose

    Middle entry clone with photoconvertible eos conjugated to a nuclear localization signal

    Depositor

    David Raible

    Article

    Curran et al Dev Biol. 2010 Aug 1;344(1):107-18. doi:

    Insert

    nls-eos
    

    Use

    Middle entry clone

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rap-MESA-20-M-Gal4

    Plasmid

    #84554

    Purpose

    MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

    Depositor

    Joshua Leonard

    Article

    Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

    Insert

    Rap-MESA-FRB-20-M-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pRDA_275

    Plasmid

    #179091

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    Cas9-NG
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046538

    Plasmid

    #149128

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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