We narrowed to 26,403 results for: Vit

1. pHIV-EGFP.Flag-EZH2-mt1

   Plasmid

   #220244

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 1 (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R27A, R31A, F165A

   Promoter

   EF-1α

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. GPR182-DuET

   Plasmid

   #213272

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPR182 (GPR182 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. EDNRA-DuET

   Plasmid

   #213232

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   EDNRA (EDNRA Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-HF1-plus

   Plasmid

   #126768

   Purpose

   Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; Q695A; Q926A; amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GRM2-DuET

   Plasmid

   #213301

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GRM2 (GRM2 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYO1031

   Plasmid

   #235706

   Purpose

   Expression of FL human Beclin 1 and FL human UVRAG in mammalian cells

   Depositor

   Roger Williams

   Article

   Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.755

   Inserts

   Beclin 1 (BECN1 Human)
   UVRAG (UVRAG Human)
   

   Expression

   Mammalian

   Promoter

   CAG and CMV

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST TFR1

   Plasmid

   #169890

   Purpose

   Express TFR1 open reading frame. Lacks IRE regulatory elements

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   TFRC (TFRC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   TFRC cDNA, lacking 3'UTR regulatory elements

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCR5-DuET

   Plasmid

   #213201

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR5 (CCR5 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Circular 200,100 (RAB7A)

   Plasmid

   #170118

   Purpose

   AAV vector carrying a guide RNA targeting the human RAB7A mRNA

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (RAB7A Human)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pMega-MaFRSA

    Plasmid

    #200226

    Purpose

    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

    Depositor

    Alanna Schepartz

    Article

    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

    Inserts

    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    mutated Asparagine 166 to Alanine and Valine 168 …

    Promoter

    proK-lacO and tacI

    Available Since

    June 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1GFP-Kir7.1

    Plasmid

    #79118

    Purpose

    Express N-terminal fused Kir7.1 protein in mammalian cells

    Depositor

    Bikash Pattnaik

    Article

    Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi:

    Insert

    Inwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CIRTS-control

    Plasmid

    #213788

    Purpose

    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

    Insert

    CIRTS-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn1, U6

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR12-DuET

    Plasmid

    #213260

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR12 (GPR12 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. VN-SOD1

    Plasmid

    #89757

    Purpose

    expresses human SOD1 fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    SOD1 (SOD1 Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-Eomes-3xT7

    Plasmid

    #200888

    Purpose

    Inducible lentiviral expression of Eomes

    Depositor

    Kai Ge

    Article

    Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

    Insert

    Eomes (Eomes Mouse)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    3xT7

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3xFLAG CMV14 / BRAF-D594G

    Plasmid

    #131719

    Purpose

    Mammalian Expression of Flag tagged BRAF

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

    Insert

    BRAF (BRAF Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D594G

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid

    #164903

    Purpose

    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    RaichuEV-Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    Y40C mutation in the Rac1 domain whcih make the s…

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDyn1

    Plasmid

    #64067

    Purpose

    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    DYNC1H1 (DYNC1H1 Human)
    

    Use

    Cre/Lox

    Tags

    His-ZZ-TCS-SNAPf

    Expression

    Insect

    Mutation

    Codon optimized for Sf9

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28b-ptetO::bac::gfp

    Plasmid

    #217882

    Purpose

    expresses the bac-BGC in bacterial cells, produces bacillamide D

    Depositor

    Tobias A. M. Gulder

    Article

    Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

    Inserts

    Dehydrogenase
    Non-ribosomal peptide synthetase
    Aminotransferase
    

    Expression

    Bacterial

    Promoter

    ptetO

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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