We narrowed to 19,741 results for: Cre

1. Met138ILeu ELL2

   Plasmid

   #127260

   Purpose

   For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met138 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Met186ILeu ELL2

   Plasmid

   #127261

   Purpose

   For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met186 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met186 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Met138ILeu, Met186ILeu ELL2

   Plasmid

   #127265

   Purpose

   For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Two Mets (138,186) to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Met133ILeu ELL2

   Plasmid

   #127259

   Purpose

   For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met133 to Ileu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Met133ILeu, Met186ILeu ELL2

   Plasmid

   #127264

   Purpose

   For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Two Mets (133,186 )to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Met331Leu ELL2

   Plasmid

   #127262

   Purpose

   For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met331 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met331 to ILeu Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. COOHend ELL2

   Plasmid

   #127255

   Purpose

   Expresses the C terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (C-terminal end) (ELL2 Human)
   

   Use

   Other

   Expression

   Mammalian

   Mutation

   contains only the last 389 aa

   Promoter

   pEF1a

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Met1ILeu ELL2

   Plasmid

   #127258

   Purpose

   For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met1 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   Met1 to Ileu by Quikchange

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ELL2mRNA+polyA

   Plasmid

   #127256

   Purpose

   For in vitro translation of human ELL2 with 30 nt pA tail

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Mutation

   shortens 3'UTR to 40 bp adds A30 tail

   Promoter

   T7

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NH2end ELL2

    Plasmid

    #127252

    Purpose

    Expresses the N terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (1-287) (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    contains only amino acids 1-289

    Promoter

    pEF1a

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

12. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

    Plasmid

    #121836

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid

    #121837

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

    Plasmid

    #121838

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid

    #121840

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JN511: pMVP (L3-L2) pA; EF1a::eGFP-P2A-TETa-pA

    Plasmid

    #121803

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + EF1a::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. IG806: pMVP (L3-L2) pA; RIP::eGFP-P2A-TETa-pA

    Plasmid

    #121807

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + RIP::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LF901: pMAGIC (L1-R5) mU6::SaCas9 gRNA scaffold

    Plasmid

    #121811

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JI601: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121815

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JI702: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; RIP

    Plasmid

    #121820

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and RIP for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge