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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,029 results for: LEA

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Showing: 10981 - 11000 of 12029 results

  5. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147260-plasmid-map-sequence-id-289707

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  10. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

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  12. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

    Plasmid
    #147279
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147279-plasmid-map-sequence-id-355936

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  13. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid
    #179526
    Purpose
    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 KES(1107-1109)GG
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    replaced KES (residues 1107-1109) with GG
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179526-plasmid-map-sequence-id-351748

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  20. pJK528

    Plasmid
    #155388
    Purpose
    dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Available Since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    155388-plasmid-map-sequence-id-324484

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Showing: 10981 - 11000 of 12029 results