We narrowed to 16,185 results for: LEA
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Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only
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pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialPromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b-His-RF2
Plasmid#124130PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
5UAS zfSynaptophysin:GFP
Plasmid#74316PurposeA fusion of zebrafish synaptophysin to GFP under the control of 5UAS repeatsDepositorInsertsynaptophysin
TagsEGFPMutationQ12H and deletion of F13 (please see depositor co…Promoter5UASAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec318
Plasmid#130871PurposeA nuclear plasmid encoding the WT TP-DNAP1 for OrthoRepDepositorInsertTP-DNAP1
ExpressionYeastAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19ExpressionBacterialPromoterlacAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
mPiezo2-CMV-Sport6
Plasmid#81073Purposemouse Piezo2DepositorInsertPiezo2 (please see depositor comments below) (Piezo2 Mouse)
ExpressionMammalianAvailable SinceNov. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BAF
Plasmid#172447PurposeExpression of nuclear protein BAF with an N-terminal mCherry tagDepositorInsertBAF
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyCatcher
Plasmid#72326PurposeBacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.DepositorInsertAviTag-SpyCatcher
TagsN-term His6 tag and TEV cleavage siteExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
TagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…Available SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
Tagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM5505
Plasmid#132664PurposepRL623-based helper plasmid for conjugations, with RSF1010 mobA geneDepositorInsertMobA
ExpressionBacterialMutationplease see depositor comments belowPromoterp1,p3Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb
Plasmid#175544PurposeExpression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag9-myc_PDGFRtmb
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
5KO9 (HMCES)
Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
Tags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialPromoterT7Available SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330S-2-PITCh
Plasmid#63670PurposeExpresses Cas9 nuclease and the PITCh-gRNADepositorInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
TagsStrepII, TEVExpressionMammalianAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-29xGGGGCC-12xMS2
Plasmid#99149PurposeEncodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vector. *Please see comments section below about the number of verified repeats.DepositorInsert29xGGGGCC 12xMS2 hairpins
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZipGFP-Casp3
Plasmid#81241PurposeA ZipGFP-based Caspase-3 protease reporter T2A mCherryDepositorInsertZipGFP with caspase cleavage sequence T2A mCherry
ExpressionMammalianAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbAW4k-loxP-TT-loxP-mRFP1
Plasmid#134405PurposeFluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.DepositorInsertW4-loxP-TT-loxP-mRFP1
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterW4 (modified T7A1)Available SinceJan. 17, 2020AvailabilityAcademic Institutions and Nonprofits only