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FCHo1-pmCherryC1

Plasmid

#27690

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo1 (Fcho1 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
CAG-NeuroD6iresGFP

Plasmid

#45027

Depositor

Connie Cepko

Article

Cherry et al J Neurosci. 2011 May 18;31(20):7365-79.

Insert

NeuroD6 (Neurod6 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
RV-mCD4-HH5-5

Plasmid

#124985

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-5 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-5 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2_8xCTS-ECFP

Plasmid

#200249

Purpose

Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

8xCTS-ECFP

Use

Zebrafish expression

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRS416-PTEF1-MCP-no_intron

Plasmid

#127597

Purpose

low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

Depositor

David Bartel

Article

Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

Insert

1xFLAG-MCP expression cassette

Expression

Yeast

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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RhoG

Plasmid

#55694

Purpose

expression of mouse RhoG

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoG (Rhog Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
TR-Csy4

Plasmid

#80601

Purpose

Expresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

Depositor

Aravind Asokan

Article

Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

Insert

Csy4

Use

AAV

Expression

Mammalian

Promoter

CBA

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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human K19

Plasmid

#14744

Depositor

M Bishr Omary

Article

Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

Insert

Keratin 19 (KRT19 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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MK1283

Plasmid

#71428

Purpose

This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

Depositor

Howard Salis

Article

Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

Insert

Mixed Feedback Loop version of the UBER system

Mutation

T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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YIplac211-ABP1-iGFPx6

Plasmid

#177099

Purpose

Expresses Abp1-iGFPx6 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ABP1 (ABP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-kanMX6

Plasmid

#89067

Purpose

genomic GBP epitope C-terminal tagging in S. pombe

Depositor

Quanwen Jin

Article

Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

GBP

Expression

Yeast

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC124-Halo

Plasmid

#175328

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC124 (CCDC124 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Expression

Mammalian

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP23-1A(sfGFP)

Plasmid

#126970

Purpose

Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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NRAS

Plasmid

#55654

Purpose

expression of mouse NRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NRAS (Nras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Scr shRNA

Plasmid

#59299

Purpose

non-targeting shRNA

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Scr shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pOz-humBirA-GFP

Plasmid

#133283

Purpose

Expression of BirA-GFP fusions in mammalian cells

Depositor

Arman Kulyyassov

Article

Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

Insert

eGFP

Use

Retroviral

Tags

Humanized wild type biotin ligase BirA

Expression

Mammalian

Promoter

MoMuLV

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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