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pCRISPR3_BC5

Plasmid

#191860

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC4

Plasmid

#191859

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC3

Plasmid

#191858

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPR3_BC1

Plasmid

#191856

Purpose

Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

Depositor

Kimberly Reynolds

Article

Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLZRS-panTRK-CR1-EGFP

Plasmid

#187194

Purpose

To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

Depositor

Pantelis Tsoulfas

Article

O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

Insert

CR1 pan-TRK receptor

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

mo-MLV or MMLV

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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LLP728_L2_pV01_0I_FRT-TB-rLUC_TCTP-fLUC

Plasmid

#192371

Purpose

To test for the repressive potential of the Transcription Block (TB) with the FRT sites around it.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-TB-FRT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Tags

mCherry- AID

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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px459-H3.1 (Hist1h3g) sgRNA

Plasmid

#186930

Purpose

Hist1h3g sgRNA used for genomic targeting at Hist1h3g C-terminal

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hist1h3g sgRNA (H3c8 Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP30-CFP

Plasmid

#178304

Purpose

CoTrIP plasmid with HSP30 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP30pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP30 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-HSP26-CFP

Plasmid

#178306

Purpose

CoTrIP plasmid with HSP26 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

HSP26pr-CFP-tAdh1

Expression

Yeast

Promoter

HSP26 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Expression

Yeast

Promoter

GLC3 and NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx4_Rap1

Plasmid

#184662

Purpose

Mammalian expression of mouse Rap1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

rap1

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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p8291 MSCV-Neo C-HA 18E7 ΔDLLC

Plasmid

#184523

Purpose

Expression of HPV18 E7 ΔDLLC

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

HPV18 E7 ΔDLLC

Use

Retroviral

Tags

HA

Mutation

deletion of DLLC

Promoter

MSCV LTR

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pHBT-Mb(GDx-Clo_L10)

Plasmid

#183406

Purpose

Expresses Monobody GDx-Clo_L10 in E. coli

Depositor

Shohei Koide

Article

Kermani et al Nat Commun. 2020 Nov 27;11(1):6064. doi:

Insert

His tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)

Tags

His tag-Avi tag-TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#176881

Purpose

MoClo Level 0 plasmid, contains 35S

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

35S terminator

Expression

Plant

Mutation

WT

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Tags

2XGFP

Expression

Worm

Promoter

nlf-1

Available Since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2437

Plasmid

#179780

Purpose

Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF

Depositor

Mei Zhen

Article

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1 (nlf-1 Nematode)

Tags

no

Expression

Worm

Promoter

Prgef-1

Available Since

March 2, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMCL8

Plasmid

#176184

Purpose

gRNA cloning cassette with RPR1p-tetO promoter and terminator RPT1t, RPR1p-tetO-GFP-scRNA-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMCL8_28bp

Plasmid

#176185

Purpose

plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI1up

Plasmid

#176166

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-1

Use

Synthetic Biology

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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