We narrowed to 14,350 results for: flag
-
Plasmid#120299PurposeAAV vector for expression of AcrIIA4 with two miR-1 binding sitesDepositorInsertAcrIIA4-2xmiR-1 binding sites
UseAAV and CRISPRTagsFLAGExpressionMammalianAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1_CR
Plasmid#108112PurposeLentiviral expression plasmid of human LKB1 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationchange cytosine 333 to alanine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
HeFm1SpCas9
Plasmid#92110PurposeExpression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)DepositorInsert3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationR661A, Q695A, K848A, Q926A, K1003A, R1060APromoterCbhAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK4387 - human expression plasmid for SpCas9 Cluster 5
Plasmid#101191PurposeHuman expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster5(K918A/V922A/R925A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationK918A/V922A/R925APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3645 - human expression plasmid for SpCas9 Cluster 3
Plasmid#101187PurposeHuman expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster3(T657A/G658A/W659A/R661A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationT657A/G658A/W659A/R661APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MMW3770 - human expression plasmid for SpCas9 Cluster 4
Plasmid#101189PurposeHuman expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAGDepositorInserthSpCas9-Cluster4(F491A/M495A/T496A/N497A)
Tags3x FLAG and NLS (SV40)ExpressionMammalianMutationF491A/M495A/T496A/N497APromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXB6-WG-MSCV
Plasmid#20993DepositorInsertHOXB6 (HOXB6 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationW->G mutation in YPWM interaction motif for PB…Available SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_low
Plasmid#128851PurposeLow copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD441_WT_hi
Plasmid#128857PurposeHigh copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_hi
Plasmid#128850PurposeHigh copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_med
Plasmid#128856PurposeMedium copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ291_WT_low
Plasmid#128855PurposeLow copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_med
Plasmid#128852PurposeMedium copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_med
Plasmid#128849PurposeMedium copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ211_rha_WT_hi
Plasmid#128853PurposeHigh copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
ADORA1-Tango
Plasmid#66209PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ
Plasmid#64875Purposelentiviral expression of human POLQDepositorAvailable SinceAug. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-EGFP
Plasmid#63592PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.DepositorInsertsCas9
EGFP
UseCRISPR and LentiviralTagsFLAG and P2AExpressionMammalianPromoterEFS-NSAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only