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pDEST17-His10-Smt3-DDX5(kf)-SNAP

Plasmid

#166149

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10-Smt3 and SNAP

Expression

Bacterial

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-His10-DDX5(kf)-SNAP

Plasmid

#166152

Purpose

Expression of N. furzeri DDX5 in E. coli

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

DDX5

Tags

His10 and SNAP

Expression

Bacterial

Promoter

T7

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-EGFP

Plasmid

#166137

Purpose

EGFP expression under the control of the GPD promoter yeast

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

EGFP

Expression

Yeast

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-mRuby3

Plasmid

#166138

Purpose

mRuby3 expression under control of the GPD promoter in yeast

Depositor

Daniel Jarosz

Publication

Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

Insert

mRuby3

Expression

Yeast

Promoter

GDP

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

Plasmid

#207050

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

P85L P86L

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

Plasmid

#207051

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

T56A R65A T87A

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

Plasmid

#207052

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85A P86A

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

Plasmid

#207053

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85L P86L

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

Plasmid

#207054

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Mutation

L55W T56A V61W R65A

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xVSV

Expression

Yeast

Promoter

pATG8

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Publication

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.23_Promoter_5

Plasmid

#210632

Purpose

Luciferase experiment for TAL1 promoter 5

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

promoter 5 (TAL1 Human)

Expression

Mammalian

Promoter

Minimal Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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IAA17-4flag-HIS5

Plasmid

#207015

Purpose

For ORF tagging with IAA17-4Flag. IAA17 is an auxin-inducible degron (AID).

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-HIS5

Plasmid

#207014

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker HIS5 (Schizosaccharomyces Pombe)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-MET15

Plasmid

#207013

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker MET15 (Saccharomyces cerevisiae)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-URA3

Plasmid

#207012

Purpose

Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_1

Plasmid

#210625

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Publication

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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