We narrowed to 15,769 results for: LEA

1. pVITRO1-TagRFP-LaminB1

   Plasmid

   #184448

   Purpose

   Nuclear memebrane-targeted TagRFP expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   TagRFP-LaminB1
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNUT2

   Plasmid

   #175745

   Purpose

   Single-component photo-inducible (LOV2-based) nucleolus-targeting tool 2

   Depositor

   Yubin Zhou

   Article

   Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

   Insert

   mCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS1018-Tier1-PhCMV-p2A

   Plasmid

   #169491

   Purpose

   Tier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven 2A sequence of porcine teschovirus-1
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pVITRO1-mCardinal-LaminB1

   Plasmid

   #184452

   Purpose

   Nuclear memebrane-targeted mCardinal expression in mammalian cells

   Depositor

   Chuan-Hsiang Huang

   Article

   Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

   Insert

   mCardinal-LaminB1
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Rat EF1α

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. H2B-HT-mGold

   Plasmid

   #183988

   Purpose

   Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

   Depositor

   Pablo Rivera-Fuentes

   Article

   Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

   Insert

   H2B (H2BC11 Human)
   

   Tags

   HaloTag, mGold

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAPV-FLAG-DsRed2-ER-5

   Plasmid

   #170620

   Purpose

   Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   APV-FLAG-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPV-FLAG-DsRed2-ER-5

   Plasmid

   #170621

   Purpose

   Expresses FLAG-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

   Depositor

   Ke Xu

   Article

   Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

   Insert

   EPV-FLAG-DsRed2-ER-5
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EC0_3_Mxi1

   Plasmid

   #163715

   Purpose

   Plasmid encoding Mxi1 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Insert

   Mxi1+SV40 NLS
   

   Use

   Synthetic Biology; Moclo vector level 0 (type 4a)

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWSK29-wzzBST

   Plasmid

   #172968

   Purpose

   pWSK29 with Salmonella typhimurium wzzBST gene

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Insert

   wzzB gene from Salmonella enterica serovar Typhimurium
   

   Expression

   Bacterial

   Mutation

   K198E- please see depositor comments

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pWSK29-fepEST

    Plasmid

    #172969

    Purpose

    pWSK29 with Salmonella typhimurium fepEST gene

    Depositor

    Renato Morona

    Article

    Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

    Insert

    fepE gene from Salmonella enterica serovar Typhimurium
    

    Expression

    Bacterial

    Mutation

    G160S- please see depositor comments

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPQD0CM0548

    Plasmid

    #162299

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CM0545

    Plasmid

    #162296

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0CM0542

    Plasmid

    #162293

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-GST-[thrombin cleavage site]
    

    Use

    Synthetic Biology

    Mutation

    codon optimized for E. coli

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Synapsin-mTurquoise-3XNLS-WPRE

    Plasmid

    #159949

    Purpose

    optical regulation of nuclear export

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mTurquoise-3XNLS
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pISH-Olfr1395

    Plasmid

    #136731

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1395

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Olfr1395
    

    Use

    In situ probe

    Mutation

    ISH Probe insert, D175G and L220F- please dep. co…

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2040

    Plasmid

    #139930

    Purpose

    Complementation plasmid for LetAB with LetB TM region replaced by PelB leader sequence

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Expression

    Bacterial

    Mutation

    LetA-(PelB-LetB(43-877))-6xHis

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046245

    Plasmid

    #149142

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046243

    Plasmid

    #149143

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF8 protein (ORF8 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046240

    Plasmid

    #149144

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046547

    Plasmid

    #149145

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge