We narrowed to 4,630 results for: pcr
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Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6
Plasmid#219694PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG4-PhiC31B-Bxb1B-GG6
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I75_tUBQ10_GG5-GG6
Plasmid#219714PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG5-tUBQ10-GG6
UseSynthetic BiologyMutationWT terminator with added golden gate linkersAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6
Plasmid#219693PurposeLevel 0 to construct history-dependent target T2PBDepositorInsertGG4-Bxb1B-PhiC31B-GG6
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLXIN2-RFP
Plasmid#99205PurposeRFP expressing bicistronic retroviral vectorDepositorInsertRed fluorescent protein
UseRetroviral; Bicistronic retroviral expression vec…ExpressionMammalianMutationThe dsRed cDNA was PCR amplified off AddGene plas…Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-SRM (sortase recognition motif)-kanMX6
Plasmid#54489PurposePCR based C-terminal SRM tagging in yeastDepositorInsertSRM (sortase-recognition motif)
UseYeast genome targetingTagskanMX6Available SinceAug. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6
Plasmid#219712PurposeLevel 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.DepositorInsertGG4-pGATA23-Bxb1-tUBQ10-GG6
UseSynthetic BiologyAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2
Plasmid#219692PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG1-PhiC31P-bbs1-BxB1P-GG2
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I89-pMUTE_GG0-1
Plasmid#219708PurposeLevel 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pMUTE-GG1
UseSynthetic BiologyMutationWT promoter with added golden gate linkersAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I74-_tUBQ10_GG4-GG6
Plasmid#219713PurposeLevel 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG4-tUBQ10-GG6
UseSynthetic BiologyMutationWT terminator with added golden gate linkersAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4
Plasmid#219709PurposeLevel 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pSPCH:PhiC31:tUBQ1-GG4
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I87-SPCHPromoter_GG0-1
Plasmid#219707PurposeLevel 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pSPCH-GG1
UseSynthetic BiologyMutationWT promoter with added golden gate linkersAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyMutationWT promoter with added golden gate linkersAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only