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We narrowed to 25,859 results for: nes

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Showing: 11181 - 11200 of 25859 results

  3. 3xFLAG-dCas9/p-bacteria

    Plasmid
    #64325
    Purpose
    Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do
    Insert
    3xFLAG-dCas9
    Use
    CRISPR; Tetracycline-inducible
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pLtetO-1
    Available Since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    64325-plasmid-map-sequence-id-107969

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  5. pUD-MSP1D1

    Plasmid
    #161608
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1; all tryptophans and tyrosines w…
    Promoter
    T7
    Available Since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161608-plasmid-map-sequence-id-317655

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  6. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid
    #205151
    Purpose
    Express UbC-driven LARGE1 and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205151-plasmid-map-sequence-id-405996

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  18. pRCC-K

    Plasmid
    #81191
    Purpose
    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below. and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81191-plasmid-map-sequence-id-154417

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  20. pRDA_118

    Plasmid
    #133459
    Purpose
    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133459-plasmid-map-sequence-id-261403

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Showing: 11181 - 11200 of 25859 results