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pLenti CMV Puro DEST (w118-1) FAM149A

Plasmid

#107510

Purpose

Expressing FAM149a, puromycin resistant

Depositor

Alejandro Gutierrez

Publication

Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

Insert

FAM149A (FAM149A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.S72A

Plasmid

#106197

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

GFAP

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.A184V

Plasmid

#106196

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

GFAP

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pECFB1_LIC-empty

Plasmid

#258243

Purpose

pFastBac1 derivative with the pMCSG7 ligation-independent cloning (LIC) site allowing bacmid production for a 6xHisTEV tagged protein

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Type

Empty backbone

Tags

6xHis-TEV

Expression

Insect

Available since

July 29, 2026

Availability

Academic Institutions and Nonprofits only

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pK-Cas9-tracr-L

Plasmid

#230860

Purpose

Long tracrRNA (tracr-L) from Streptococcus pyogenes which acts as a natural single guide RNA

Depositor

Kutubuddin Molla

Publication

Karmakar et al Plant Cell Rep. 2024 Nov 23;43(12):291.

Insert

Long tracrRNA (tracr-L) from S. pyogenes replaces gRNA scaffold

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available since

July 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLIX403_HA-SNRPB_RtoA

Plasmid

#258256

Purpose

Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPB with arginine-to-alanine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

arginine-to-alanine

Promoter

TRE promoter, Tet ON

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLIX403_HA-SNRPB_RtoAx2

Plasmid

#258257

Purpose

Lentiviral inducible expression of N-terminal HA-tagged human SNRPB with two arginine-to-alanine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

R107 and R111 arginine-to-alanine

Promoter

TRE promoter, Tet ON

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pECFB1_HsMEP50

Plasmid

#258245

Purpose

Baculovirus/insect cell expression of human MEP50/WDR77 with an N-terminal cleavable His tag

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

WDR77 (WDR77 Human)

Tags

6xHis-TEV

Expression

Insect

Promoter

polyhedrin

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pECFB1_HsPRMT5

Plasmid

#258244

Purpose

Baculovirus/insect cell expression of human PRMT5 with an N-terminal cleavable His tag

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

PRMT5 (PRMT5 Human)

Tags

6xHis-TEV

Expression

Insect

Promoter

polyhedrin

Available since

July 8, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPD3_RtoK

Plasmid

#258279

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPD3 with arginine-to-lysine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-lysine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPD1_RtoK

Plasmid

#258282

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPD1 with arginine-to-lysine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD1 (SNRPD1 Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-lysine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPD3_RtoA

Plasmid

#258280

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPD3 with arginine-to-alanine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-alanine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPD1_RtoA

Plasmid

#258283

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPD1 with arginine-to-alanine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD1 (SNRPD1 Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-alanine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPB_RtoK

Plasmid

#258276

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPB with arginine-to-lysine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-lysine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR221_HA-SNRPB_RtoA

Plasmid

#258277

Purpose

Gateway entry plasmid for N-terminal HA-tagged human SNRPB with arginine-to-alanine mutations

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPB (SNRPB Human)

Use

Gateway Cloning

Tags

HA

Mutation

arginine-to-alanine

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLIX403_HA-SNRPD3_RtoK

Plasmid

#258264

Purpose

Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-lysine substitutions

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

arginine-to-lysine

Promoter

TRE promoter, Tet ON

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLIX403_HA-SNRPD3_RtoA

Plasmid

#258265

Purpose

Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD3 with arginine-to-alanine substitutions

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD3 (SNRPD3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

arginine-to-alanine

Promoter

TRE promoter, Tet ON

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLIX403_HA-SNRPD1_RtoK

Plasmid

#258267

Purpose

Tet-inducible lentivirus expression of N-terminal HA-tagged human SNRPD1 with arginine-to-lysine substitutions

Depositor

David Shechter

Publication

DeAngelo et al Mol Cell. 2025 Nov 6;85(21):4016-4031.e9

Insert

SNRPD1 (SNRPD1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

arginine-to-lysine

Promoter

TRE promoter, Tet ON

Available since

July 2, 2026

Availability

Academic Institutions and Nonprofits only

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pIRES2-AcGFP1-PX-Gal-3-TRUNC

Plasmid

#256299

Purpose

Expresses truncated PX-Gal-3 (deletion of N-terminal domain, aa 1-116) in mammalian cells

Depositor

Mia Huang

Publication

Joeh et al Proc Natl Acad Sci U S A. 2020 Nov 3;117

Insert

PX-Gal-3-TRUNC (LGALS3 Human)

Tags

His tag

Expression

Mammalian

Mutation

Deleted residues 1-116

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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