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pStA0::SPLc17-RBSc123

Plasmid

#171912

Purpose

Promoter SPLc17 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc17 and RBSc123

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc21

Plasmid

#171884

Purpose

Promoter SPLc3 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab37

Plasmid

#140886

Purpose

Expression of zebrafish GFP-tagged Rab37 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab37

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_stgRNA-Puro

Plasmid

#164124

Purpose

Backbone for lentiviral stgRNA library construction (library 1 and 2)

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

human U6

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP35-TD-HilA

Plasmid

#153332

Purpose

Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBz118_His_CsoS2

Plasmid

#140841

Purpose

Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz55_polyPro_random_Npeptide

Plasmid

#140846

Purpose

Same as pLz47 but with randomized N*-peptide sequence

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with randomized CsoS2 N-peptide

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz57_polyPro_CsoS2_CTD_r8

Plasmid

#140849

Purpose

Same as pLz47 but with CTD repeat 8 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 CTD repeat 8

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP38-1A(sfGFP)

Plasmid

#126984

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). Origin pUC (high copy number). sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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mTalin2_Neck

Plasmid

#111032

Purpose

Plasmid expresses the unstructured neck region of mouse talin2

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R3

Plasmid

#111035

Purpose

Plasmid expresses the talin2 rod sub domain R3 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R2

Plasmid

#111034

Purpose

Plasmid expresses the talin2 rod sub domain R2 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R12

Plasmid

#111045

Purpose

Plasmid expresses the talin2 rod domain R12 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R5

Plasmid

#111037

Purpose

Plasmid expresses the talin2 rod domain R5 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_F1

Plasmid

#111029

Purpose

Plasmid expresses mouse talin2 FERM domain F1

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13

Plasmid

#111046

Purpose

Plasmid expresses the talin2 rod domain R13 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13-DD

Plasmid

#111047

Purpose

Plasmid expresses the talin2 rod domain R13 and the dimerisation domian DD.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_F0

Plasmid

#111028

Purpose

Plasmid expresses mouse talin2 FERM domain F0

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R4

Plasmid

#111036

Purpose

Plasmid expresses the talin2 rod sub domain R4 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge