We narrowed to 11,647 results for: emb

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pLV[Exp]-EF1A>3xFLAG/3xGGGGS /mMboat2[NM_026037.3]

Plasmid

#258331

Purpose

Expresses mouse Mboat2 [NM_026037.3] with 3XFLAG in a lentiviral expression vector

Depositor

Xiaoai Zhao

Publication

Zhao et al Sci Adv. 2026 Jul 31;12(31):eaeh9771. do

Insert

Mboat2 (Mboat2 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1α

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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MTS-TWINKLE(IDR)-mCh-CRY2olig

Plasmid

#254613

Purpose

Expresses mt-optoIDR with TWINKLE (1-170 aa) to optogenetically induce biomolecular condensates within mitochondrial matrix in living cells.

Depositor

Marina Feric

Publication

Aththawala Gedara et al Commun Biol. 2026 Apr 23. doi: 10.1038/s

Insert

TWNK (TWNK Human)

Use

Synthetic Biology

Tags

mCherry-Cry2olig

Expression

Mammalian

Available since

May 1, 2026

Availability

Academic Institutions and Nonprofits only

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SSH-3 WT

Plasmid

#245957

Purpose

Expression of wild-type slingshot 3, a cofilin pSer3 phosphatase

Depositor

James Bamburg, Kensaku Mizuno, Kazumasa Ohashi

Publication

Ohta et al Genes Cells. 2003 Oct;8(10):811-24. doi:

Insert

Slingshot protein phosphatase 3 (Ssh3 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Publication

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM1 WPRE

Plasmid

#236233

Purpose

AAV expression of mouse STIM1 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Publication

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Stim1 (Stim1 Mouse)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1-Y414A

Plasmid

#222320

Purpose

Synchronize the trafficking of LAMP1-A17 from the ER.

Depositor

Franck Perez

Publication

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB:GSlinker:SSD

Plasmid

#228535

Purpose

FRB protein in fusion with a bipartite SSD degron, consisting of fragments of human Skp1 and SOCS2 proteins, through a flexible glycine-serine linker.

Depositor

Roman Jerala

Publication

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

FRB:GSlinker:SSD (SKP1, SOCS2 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCHC023 (∆MBH)

Plasmid

#226201

Purpose

Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

Depositor

Christopher Johnson

Publication

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for membrane bound hydrogenase operon

Use

Synthetic Biology

Mutation

∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

Available since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPICZ A-Strep-Opa1(MGD) (SB252)

Plasmid

#227601

Purpose

Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

Depositor

Luke Chao

Publication

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

OPA1 (OPA1 Human)

Tags

Twin-StrepTag

Expression

Yeast

Mutation

contains aa 253-580 (MGD) and aa 938-960

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Actin-Vhh-sfGFP-P2A-1xHA-iRFP-caax (JDW 1216)

Plasmid

#224496

Purpose

Gateway compatible middle entry clone containing an Actin nanobody fused to sfGFP followed by P2A and iRFP-caax tag (GFP actin reporter and cell membrane iRFP reporter)

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-sfGFP-P2A-HA-iRFP-caax

Use

Gateway Cloning

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ:K2P2.1(mTREK-1)Cryst S112C

Plasmid

#224780

Purpose

Pichia pastoris expression vector for generating mouse TREK-1(21S-322T) + 3C cleavable site + eGFP + 10x His under the AOX1 promoter

Depositor

Dan Minor

Publication

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily K member 2 (Kcnk2 Mouse)

Expression

Yeast

Mutation

K84R / Q85E / T86K / I88L / A89R / Q90A / A92P / …

Promoter

AOX1 promoter

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-SpyTag003

Plasmid

#216293

Purpose

Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

Depositor

Mark Howarth

Publication

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-cPLA2 C2 domain (Y96C)

Plasmid

#213633

Purpose

Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

cPLA2 C2 domain (Y96C) (PLA2G4A Human)

Tags

SUMO

Expression

Bacterial

Mutation

Y96C, C139A, C141S

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV Flag-LRRK2 K17A/K18A/R1441G

Plasmid

#186013

Purpose

Flag-LRRK2 K17A/K18A/R1441G

Depositor

Suzanne Pfeffer

Publication

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

Flag-LRRK2 K17A/K18A/R1441G (LRRK2 Human)

Expression

Mammalian

Mutation

See depositor's comments below.

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1-del1844-2133-V1251R_S

Plasmid

#147484

Purpose

Mammalian Expression of HsNot1iso1-del1844-2133-V1251R

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso1-del1844-2133-V1251R (CNOT1 Human)

Expression

Mammalian

Mutation

Deletion of 5 AA compared to the sequence given b…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2 NCmut-msfGFP

Plasmid

#180311

Purpose

bacterial expression of human SEPT2 NCmut fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Publication

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Mutation

SEPT2 F20D, V27D

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-MS2-HA-C1-HsGIGYF2_1-718_AI

Plasmid

#148960

Purpose

Mammalian Expression of HsGIGYF2_1-718

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2_1-718 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2-W288A_AI

Plasmid

#148953

Purpose

Mammalian Expression of HsGIGYF2-W288A

Depositor

Elisa Izaurralde

Publication

Peter et al Genes Dev. 2019 Oct 1;33(19-20):1355-136

Insert

HsGIGYF2-W288A (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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